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Noninvasive in vivo 3D bioprinting

2020; American Association for the Advancement of Science; Volume: 6; Issue: 23 Linguagem: Inglês

10.1126/sciadv.aba7406

2375-2548

Yuwen Chen, Jiumeng Zhang, Xuan Liu, Shuai Wang, Jie Tao, Yulan Huang, Wenbi Wu, Li Yang, Kai Zhou, Xiawei Wei, Shaochen Chen, Xiang Li, Xuewen Xu, Ludwig Cardon, Zhiyong Qian, Maling Gou,

Innovative Microfluidic and Catalytic Techniques Innovation

Three-dimensional (3D) printing technology has great potential in advancing clinical medicine. Currently, the in vivo application strategies for 3D-printed macroscale products are limited to surgical implantation or in situ 3D printing at the exposed trauma, both requiring exposure of the application site. Here, we show a digital near-infrared (NIR) photopolymerization (DNP)-based 3D printing technology that enables the noninvasive in vivo 3D bioprinting of tissue constructs. In this technology, the NIR is modulated into customized pattern by a digital micromirror device, and dynamically projected for spatially inducing the polymerization of monomer solutions. By ex vivo irradiation with the patterned NIR, the subcutaneously injected bioink can be noninvasively printed into customized tissue constructs in situ. Without surgery implantation, a personalized ear-like tissue constructs with chondrification and a muscle tissue repairable cell-laden conformal scaffold were obtained in vivo. This work provides a proof of concept of noninvasive in vivo 3D bioprinting.