Artigo Acesso aberto Produção Nacional Revisado por pares

Hancornia speciosa serum fraction latex stimulates the angiogenesis and extracellular matrix remodeling processes

2020; Brazilian Academy of Sciences; Volume: 92; Issue: 2 Linguagem: Inglês

10.1590/0001-3765202020190107

ISSN

1678-2690

Autores

Patrícia Lima D’Abadia, Elisa Flávia Luiz Cardoso Bailão, Ruy de Souza Lino Júnior, Matheus Gabriel de Oliveira, Vinícius Barreto da Silva, Leandra de Almeida Ribeiro Oliveira, Edemilson Cardoso da Conceição, Paulo Roberto de Melo Reis, Leonardo Luiz Borges, Pablo José Gonçalves, Luciane Madureira Almeida,

Tópico(s)

Biological Stains and Phytochemicals

Resumo

The Hancornia speciosa latex reveals angiogenic, osteogenic, and anti-inflammatory properties, which present its potential for developing of wound healing drugs; however, the latex compounds responsible for angiogenesis remain unknown. One strategy to screen these active compounds is evaluation of latex fractions. This study aimed to obtain different fractions of latex and evaluate its angiogenic activity separately using the chick chorioallantoic membrane (CAM) assay. The serum (SE) fraction was responsible for angiogenesis, which was subject to biochemical characterization and computational simulations in order to understand the contribution of H. speciosa latex in wound healing process. Our results revealed weak antioxidant potential and absence of antimicrobial activity in the SE fraction. Phytochemical analysis identified chlorogenic acids (CGA) as the main compound of SE fraction. CGA bioactivity predictions identify different molecules associated with extracellular matrix (ECM) remodeling, such as metalloproteinases, which also are overexpressed in our CAM assay experiment. Docking simulations revealed the interactions between CGA and matrix metalloproteinase 2. In conclusion, SE latex fraction stimulates angiogenesis and may influence ECM remodeling. These properties may contribute to the wound healing process, and also confirm the widespread use of this plant.

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