Produção Nacional
Revisado por pares
Melatonin regulates the expression of inflammatory cytokines, VEGF and apoptosis in diabetic retinopathy in rats
2020; Elsevier BV; Volume: 327; Linguagem: Inglês
10.1016/j.cbi.2020.109183
ISSN1872-7786
AutoresIsmaela Maria Ferreira de Melo, Cintia Giselle Martins Ferreira, Elton Hugo Lima da Silva Souza, Lécio Leone de Almeida, Fabrício Bezerra de Sá, Clóvis José Cavalcanti Lapa Neto, Matheus Vinicius Paz de Castro, Valéria Wanderley Teixeira, Álvaro Aguiar Coelho Teixeira,
Tópico(s)Advanced Glycation End Products research
ResumoThe present study analyzed whether melatonin could mediate the expression of VEGF, IL-6 and TNF-α, as well as the apoptotic index in rats with diabetic retinopathy. Fifty Wistar albino rats were divided into the following groups: GC: rats without induction of diabetes by streptozotocin; GD: rats induced to diabetes by streptozotocin and treated with placebo; GDM: rats induced to diabetes by streptozotocin and after confirmation treated with melatonin at a dose of 10 mg/kg for 20 days; GDMS: rats induced to diabetes by streptozotocin and treated simultaneously with melatonin at a dosage of 10 mg/kg for 20 days; GDI: rats induced to diabetes by streptozotocin and after confirmation treated with insulin for 20 days. Diabetes was induced by intraperitoneal injections of streptozotocin (60 mg/kg), and insulin (5 U/day) was administered subcutaneously. For apoptosis TUNEL was used, while for the analysis of VEGF, IL-6 and TNF-α. The results showed that the groups that were treated with melatonin decreased the expression of cytokines and VEGF, in addition to apoptosis. Thus, it is concluded that melatonin can regulate the expression of these factors by improving the condition of the retina in diabetic retinopathy.
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