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BIBTEX RIS

The complex microbiota of artisanal cheeses interferes in the performance of enumeration protocols for lactic acid bacteria and staphylococci

2020; Elsevier BV; Volume: 109; Linguagem: Inglês

10.1016/j.idairyj.2020.104791

1879-0143

Thaiza Teixeira de Almeida, Milimani Andretta, Letícia Rocha Ferreira, Antônio Fernandes de Carvalho, Luís Augusto Nero,

Milk Quality and Mastitis in Dairy Cows

We assessed the interference of a complex food microbiota, present in Minas artisanal cheese (MAC) produced in the Serro region, on the performance of enumeration protocols for lactic acid bacteria (LAB) and staphylococci. Initially, 140 isolates were obtained from 6 Serro MAC samples and plated for enumeration of LAB (3 variations of MRS, M17 and Petrifilm™ LAB) and staphylococci (Baird–Parker and Petrifilm™ STX); some target isolates did not grow or present atypical morphologies in their selective protocols. Then, Serro MAC samples (n = 55) were obtained and plated for LAB and staphylococci based on the same protocols: 2569 isolates were obtained from LAB and 1164 from staphylococci protocols: Petrifim™ LAB presented the highest frequencies of LAB-confirmed colonies, as Petrifilm™ STX for staphylococci (p < 0.05).