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Induction of osteogenic differentiation by demineralized and decellularized bovine extracellular matrix derived hydrogels associated with barium titanate

2020; Elsevier BV; Volume: 66; Linguagem: Inglês

10.1016/j.biologicals.2020.06.003

1095-8320

Danielle Luciana Aurora Soares do Amaral, Rafaella de Souza Salomão Zanette, Gustavo Torres de Souza, Silvioney Augusto da Silva, Jair Adriano Kopke de Aguiar, Raphael Fortes Marcomini, Antônio Márcio Resende do Carmo, Breno Valentim Nogueira, Rodolpho José da Silva Barros, Fernando de Sá Silva, Marcelo de Oliveira Santos, Michele Munk, Humberto de Mello Brandão, Carlos Magno da Costa Maranduba,

Electrospun Nanofibers in Biomedical Applications

Bone tissue-derive biomaterials have become of great interest to treat diseases of the skeletal system. Biological scaffolds of demineralized and decellularized extracellular matrices (ECM) have been developed and one of these options are ECM hydrogels derived from bovine bone. Nanomaterials may be able to regulate stem cell differentiation due to their unique physical-chemical properties. The present work aimed to evaluate the osteoinductive effects of ECM hydrogels associated with barium titanate nanoparticles (BTNP) on dental pulp cells derived from exfoliated teeth. The addition of BTNP in the ECM derived hydrogel did not affect cell proliferation and the formation of bone nodules. Furthermore, it increased the expression of bone alkaline phosphatase. The results demonstrated that the nanobiocomposites were able to promote the osteogenic differentiation, even in the absence of chemical inducing factors for osteogenic differentiation. In conclusion, bovine bone ECM hydrogel combined with BTNP presented and increased expression of markers of osteogenic differentiation in the absence of chemical inducing factors.