Quantitative estimation of scopoletin from Argyreia speciosa (L. f.) sweet by a validated high performance thin layer chromatographic method
2020; Wiley; Volume: 3; Issue: 8 Linguagem: Inglês
10.1002/sscp.202000031
ISSN2573-1815
AutoresNiraj Vyas, Raval Manan, Nidhi Patel,
Tópico(s)Pharmacological Effects of Natural Compounds
ResumoAbstract Roots of Argyreia speciosa (L. f.) Sweet ( Convolvulaceae ) are indicated in Ayurveda to treat male reproductive system function debilities. Studies have shown that methanolic extract of the roots are capable in stimulating serum testosterone content in vitro and in vivo in rats as experimental animals. Scopoletin was isolated from ethyl acetate fraction of methanolic extract. High‐performance thin‐layer chromatography method was developed for estimation of scopoletin from bioactive fraction of Argyreia speciosa (L. f.) Sweet. Samples of isolated scopoletin were applied as thin bands (8 mm) on silica gel pre‐coated on aluminum thin‐layer chromatography plates. Separation of scopoletin was achieved using mobile phase by trial‐error approach and optimized mobile phase was consisted of petroleum ether:ethyl acetate:formic acid (3:7:0.3, v/v/v). Scanning was performed in fluorescence mode at 366 nm. Evaluation was performed by linear regression using peak area. The integration was carried out and amount of scopoletin was found 2.49 ± 0.037 mg% w/w of dried weight basis of plant extract. The developed method was also validated in terms of linearity, precision, sensitivity, accuracy, specificity, and robustness as per ICH Q2(R1) guideline. This method can serve as an analytical tool for chemical evaluation of plant extract containing scopoletin.
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