Replacement of the liquid vitreus with sodium hyaluronate in monkeys
1980; Elsevier BV; Volume: 31; Issue: 1 Linguagem: Inglês
10.1016/0014-4835(80)90092-5
ISSN1096-0007
AutoresJanet L. Denlinger, Endre A. Balazs,
Tópico(s)Corneal Surgery and Treatments
ResumoLarge molecular size, sterile pyrogen-free sodium hyaluronates from various manufacturers and a special fraction of sodium hyaluronate called Healon have been used to replace the liquid vitreus of owl monkeys (Aotus trivirgatus) and rhesus monkeys (Macaca mulatta) in order to evaluate the degree of inflammatory response in the anterior chamber and vitreus. A maximum response indicated by an increased number of leucocytes and increased amount of protein in the aqueous humor and vitreus was noted at 48 hr. Only the Healon fraction was shown to produce a similar or lower inflammatory reaction postoperatively than the control operation using physiological balanced salt solution, the solvent of the sodium hyaluronate and Healon preparations. Repeated implantation of Healon seven to nine times in the same eye did not result in any increase in inflammation or any immunogenic response. Eyes in which uveitis had previously been induced by endotoxin or concacavalin A did not show any attenuated reaction to implantation of Healon. Healon also caused a significantly lower inflammation in the rat peritoneal space than other sodium hyaluronates. The monkey eye test proves to be the most sensitive and reproducible test to detect small amounts of inflammatory agents.
Referência(s)