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A CNL protein in wild emmer wheat confers powdery mildew resistance

2020; Wiley; Volume: 228; Issue: 3 Linguagem: Inglês

10.1111/nph.16761

1469-8137

Miaomiao Li, Lingli Dong, Beibei Li, Zhengzhong Wang, Jingzhong Xie, Dan Qiu, Yahui Li, Wenqi Shi, Lijun Yang, Qiuhong Wu, Yongxing Chen, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Keyu Zhu, Yiwen Li, Yan Zhang, Rongge Wang, Chengguo Yuan, Wei Liu, Dazhao Yu, Ming‐Cheng Luo, Tzion Fahima, Eviatar Nevo, Hongjie Li, Zhiyong Liu,

Plant Pathogens and Resistance

Summary Powdery mildew, a fungal disease caused by Blumeria graminis f. sp. tritici ( Bgt ), has a serious impact on wheat production. Loss of resistance in cultivars prompts a continuing search for new sources of resistance. Wild emmer wheat ( Triticum turgidum ssp. dicoccoides , WEW), the progenitor of both modern tetraploid and hexaploid wheats, harbors many powdery mildew resistance genes. We report here the positional cloning and functional characterization of Pm41 , a powdery mildew resistance gene derived from WEW, which encodes a coiled‐coil, nucleotide‐binding site and leucine‐rich repeat protein (CNL). Mutagenesis and stable genetic transformation confirmed the function of Pm41 against Bgt infection in wheat. We demonstrated that Pm41 was present at a very low frequency (1.81%) only in southern WEW populations. It was absent in other WEW populations, domesticated emmer, durum, and common wheat, suggesting that the ancestral Pm41 was restricted to its place of origin and was not incorporated into domesticated wheat. Our findings emphasize the importance of conservation and exploitation of the primary WEW gene pool, as a valuable resource for discovery of resistance genes for improvement of modern wheat cultivars.