Portal de Periódicos da CAPES

Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo

2020; American Association for the Advancement of Science; Volume: 6; Issue: 26 Linguagem: Inglês

10.1126/sciadv.aba3239

2375-2548

Eyiyemisi C. Damisah, Robert Hill, Anupama Rai, Fuyi Chen, Carla V. Rothlin, Sourav Ghosh, Jaime Grutzendler,

Neuroscience and Neuropharmacology Research

Cell death is prevalent throughout life; however, the coordinated interactions and roles of phagocytes during corpse removal in the live brain are poorly understood. We developed photochemical and viral methodologies to induce death in single cells and combined this with intravital optical imaging. This approach allowed us to track multicellular phagocytic interactions with precise spatiotemporal resolution. Astrocytes and microglia engaged with dying neurons in an orchestrated and synchronized fashion. Each glial cell played specialized roles: Astrocyte processes rapidly polarized and engulfed numerous small dendritic apoptotic bodies, while microglia migrated and engulfed the soma and apical dendrites. The relative involvement and phagocytic specialization of each glial cell was plastic and controlled by the receptor tyrosine kinase