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Nasal carriage of various staphylococcal species in small ruminant lentivirus-infected asymptomatic goats

2023; De Gruyter; Linguagem: Inglês

10.24425/pjvs.2020.133634

2300-2557

Agata Moroz, Olga Szaluś‐Jordanow, Michał Czopowicz, Klaudia Brodzik, Violetta Petroniec, Ewa Augustynowicz‐Kopeć, Anna Lutyńska, Marta Roszczynko, A Gołoś-Wójcicka, Agnieszka Korzeniowska‐Kowal, Andrzej Gamian, Marcin Mickiewicz, Tadeusz Frymus, H Petelicka, Jarosław Kaba,

Milk Quality and Mastitis in Dairy Cows

The study was carried out in Polish goat population to estimate the prevalence of the nasal cavity infection with various staphylococcal species including methicillin-resistant Staphylococcus aureus (MRSA), investigate the potential permissive role of small ruminant lentivirus (SRLV) infection and determine the level of clonality of S. aureus nasal isolates.Nasal swabs and blood samples were collected from 1300 clinically healthy adult goats from 21 Polish goat herds.Blood samples were serologically screened for SRLV.Staphylococci were isolated from nasal swabs and identified using classical microbiological methods, MALDI-TOF, multiplex-PCR, and their clonality was assessed using PFGE.Antimicrobial resistance was determined on the basis of minimum inhibitory concentration and by demonstration of the presence of the mecA gene encoding the multiplex-PCR PBP2a protein and of the five main types of staphylococcal cassette chromosome mec.The apparent prevalence of staphylococcal and S. aureus infection of the nasal cavity was 29.1% (CI 95%: 26.9%, 31.5%) and 7.3% (CI 95%: 6.1%, 8.8%), respectively.No relationship was found between the SRLV-infection and the presence of any staphylococcal species including S. aureus (p=0.143).Only 9.8% of S. aureus isolates were resistant to amoxicillin/clavulanic acid and 5.9% to chloramphenicol and ciprofloxacin.All tested isolates proved to be phenotypically and genotypically sensitive to methicillin, which yielded the apparent prevalence of MRSA of 0% (CI 95%: 0%, 7.0%).S. aureus isolates show high genetic similarity within goat herds, however vary considerably between herds.Goats do not appear to be an important source of S. aureus for humans in Poland.