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BIBTEX RIS

Leucine-Rich Diet Modulates the Metabolomic and Proteomic Profile of Skeletal Muscle during Cancer Cachexia

2020; Multidisciplinary Digital Publishing Institute; Volume: 12; Issue: 7 Linguagem: Inglês

10.3390/cancers12071880

ISSN

2072-6694

Autores

Bread Cruz, André Gustavo de Oliveira, Laís Rosa Viana, Leisa Lopes‐Aguiar, Rafael Renatino Canevarolo, Maiara Caroline Colombera, Rafael Rossi Valentim, Fernanda Garcia-Fóssa, Lizandra Maia de Sousa, Bianca Gazieri Castelucci, Sílvio Roberto Consonni, Daniel Martins‐de‐Souza, Marcelo Bispo de Jesus, Steven Thomas Russell, Maria Cristina Cintra Gomes‐Marcondes,

Tópico(s)

Diet and metabolism studies

Resumo

Background: Cancer-cachexia induces a variety of metabolic disorders, including skeletal muscle imbalance. Alternative therapy, as nutritional supplementation with leucine, shows a modulatory effect over tumour damage in vivo and in vitro. Method: Adult rats distributed into Control (C), Walker tumour-bearing (W), control fed a leucine-rich diet (L), and tumour-bearing fed a leucine-rich diet (WL) groups had the gastrocnemius muscle metabolomic and proteomic assays performed in parallel to in vitro assays. Results: W group presented an affected muscle metabolomic and proteomic profile mainly related to energy generation and carbohydrates catabolic processes, but leucine-supplemented group (WL) recovered the energy production. In vitro assay showed that cell proliferation, mitochondria number and oxygen consumption were higher under leucine effect than the tumour influence. Muscle proteomics results showed that the main affected cell component was mitochondria, leading to an impacted energy generation, including impairment in proteins of the tricarboxylic cycle and carbohydrates catabolic processes, which were modulated and improved by leucine treatment. Conclusion: In summary, we showed a beneficial effect of leucine upon mitochondria, providing information about the muscle glycolytic pathways used by this amino acid, where it can be associated with the preservation of morphometric parameters and consequent protection against the effects of cachexia.

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