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Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

2020; Wiley; Volume: 17; Issue: 9 Linguagem: Inglês

10.1002/cbdv.202000369

1612-1880

Luíza Stolz Cruz, Carla Cristine Kanunfre, Evelyn Assis de Andrade, Alexandre Alves de Oliveira, Laura Stolz Cruz, Mariane de Faria Moss, Guilherme L. Sassaki, Leociley Rocha Alencar Menezes, Mei Wang, Ikhlas Ahmad Khan, Flávio Luís Beltrame,

Biological Stains and Phytochemicals

Abstract The current study was carried out by a bioguided fractionation of a hexane extract of the latex of Euphorbia umbellata against leukemic cells. Samples were analyzed by NMR, GC/MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC/MS analysis. MTT reduction assay demonstrated that HL‐60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices were higher than 22.44. Bioguided‐fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.