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First Report of Sweet Potato Feathery Mottle Virus Infecting Chrysanthemum morifolium in China

2020; American Phytopathological Society; Volume: 104; Issue: 12 Linguagem: Inglês

10.1094/pdis-10-19-2156-pdn

1943-7692

Keru Yan, Y. H. Zhang, Chengcong Yang, C. N., Bosai He, Bizeng Mao,

Plant-Microbe Interactions and Immunity

HomePlant DiseaseVol. 104, No. 12First Report of Sweet Potato Feathery Mottle Virus Infecting Chrysanthemum morifolium in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Sweet Potato Feathery Mottle Virus Infecting Chrysanthemum morifolium in ChinaK. R. Yan, Y. H. Zhang, C. B. Yang, C. N. Ma, B. W. He, and B. Z. MaoK. R. YanInstitute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Hangzhou, 310058, ChinaSearch for more papers by this author, Y. H. ZhangInstitute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Hangzhou, 310058, ChinaSearch for more papers by this author, C. B. YangInstitute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Hangzhou, 310058, ChinaSearch for more papers by this author, C. N. MaAgricultural Technology Extension Service Center of Tongxiang City, Hangzhou, 314500, ChinaSearch for more papers by this author, B. W. HeAgricultural Technology Extension Center of Zhejiang Province, Hangzhou, 310020, ChinaSearch for more papers by this author, and B. Z. Mao†Corresponding author: B. Z. Mao; E-mail Address: maobz@zju.edu.cnInstitute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Hangzhou, 310058, ChinaSearch for more papers by this author AffiliationsAuthors and Affiliations K. R. Yan1 2 Y. H. Zhang1 2 C. B. Yang1 2 C. N. Ma3 B. W. He4 B. Z. Mao1 2 † 1Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China 2Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Hangzhou, 310058, China 3Agricultural Technology Extension Service Center of Tongxiang City, Hangzhou, 314500, China 4Agricultural Technology Extension Center of Zhejiang Province, Hangzhou, 310020, China Published Online:13 Oct 2020https://doi.org/10.1094/PDIS-10-19-2156-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleChrysanthemum (Chrysanthemum morifolium Ramat.) is one of the eight well-known traditional medicinal herbs (“zhe ba wei”) in China. Its flowers are used in the production of medicine for antitumor, antibiosis, anti-inflammatory, antioxidation, and hypolipidemic purposes in modern pharmacology (Cui et al. 2014; Lee et al. 2009; Lii et al. 2010; Wang et al. 2010). Tongxiang City in the Zhejiang Province of China is a major C. morifolium production region, with a cultivation area of 3,161 ha and an annual yield of 9,090 metric tons (Tongxiang Statistical Yearbook 2019, unpublished). In July 2018, chrysanthemum plants with symptoms of chlorotic blotches, mosaic, and mottle were observed in a greenhouse in Tongxiang City, where the disease incidence was estimated to be 100%. To reveal the possible agents responsible for these virus-like symptoms, a total of 18 plants were collected and tested first by RT-PCR for various viruses and viroids that have been reported in chrysanthemum in China in the past (Hu et al. 2018; Zhao et al. 2015), including chrysanthemum virus B, tomato aspermy virus, cucumber mosaic virus, tobacco mosaic virus, potato virus X, potato virus Y, tomato spotted wilt virus, chrysanthemum stunt viroid, and chrysanthemum chlorotic mottle viroid. Surprisingly, none of abovementioned viruses and viroids was detected. Nevertheless, a transmission electron microscopy (Hitachi H7650) assay on a representative sample detected flexuous filament-shaped virus-like particles of 600 to 900 nm in length after negative staining with phosphotungstic acid. The 18 samples were then tested using DAS-ELISA with antibodies (DSMZ, Germany) against sweet potato feathery mottle virus (SPFMV), zucchini yellow mosaic virus, and turnip mosaic virus. Only SPFMV was detected serologically in all screened samples. To confirm the ELISA results, the samples were again subjected to total RNA extraction using TRIzol Reagent (Takara) and followed by RT-PCR assay with the SPFMV-specific primer pair, SPFMV 1F (5′-TACACACTGCTAAAACTAGG-3′) and SPFMV 1R (5′-AGTTCATCATAACCCCATGA-3′) (Kwak et al. 2014). An amplicon of the predicted size (356 bp) was obtained from all the samples. The amplicon was then purified with the DNA Gel Extraction Kit (Tsingke, China), cloned into the pGEM-T Easy Vector (Promega, U.S.A.), and sequenced by the Sanger method. A consensus sequence obtained from more than eight clones originated from one sample was deposited in GenBank under accession number MH844809. BLASTn analysis indicated that the sequence shared 98.6% nucleotide identity with that of a Chinese isolate of SPFMV from Dendrobium candidum (MH508094) and a Korean isolate of SPFMV from sweet potato (KP115608). To our knowledge, this is the first report of SPFMV naturally infecting C. morifolium in China. In consideration of the wide planting area of C. morifolium in China, a SPFMV outbreak would cause serious economic losses to farmers. Therefore, it is worthwhile to prevent its spread to other chrysanthemum-cultivating regions.The author(s) declare no conflict of interest.References:Cui, Y., et al. 2014. Nutr. 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Virol. 160:1145. https://doi.org/10.1007/s00705-015-2360-z Crossref, ISI, Google ScholarAll authors in this manuscript have read and approved the current version of the article.The author(s) declare no conflict of interest.Funding: This research study was funded by the following: Ministry of Agriculture Agricultural Major Technology Collaborative Promotion Project of China (2018XTTGYC04) and Major scientific and technological projects for the selection of new varieties of Chinese herbal medicines in Zhejiang Province (2016C02058).DetailsFiguresLiterature CitedRelated Vol. 104, No. 12 December 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionUredinia of Phragmidium violaceum on European blackberry (K. J. Evans et al.). Photo credit: L. Morin. Strawberry fruit rot caused by Sclerotinia sclerotiorum (M. V. Marin and N. A. Peres). Photo credit: M. V. Marin. Metrics Downloaded 446 times Article History Issue Date: 1 Dec 2020Published: 13 Oct 2020First Look: 13 Jul 2020Accepted: 6 Jul 2020 Pages: 3273-3273 Information© 2020 The American Phytopathological SocietyFundingMinistry of Agriculture Agricultural Major Technology Collaborative Promotion Project of ChinaGrant/Award Number: 2018XTTGYC04Major scientific and technological projects for the selection of new varieties of Chinese herbal medicines in Zhejiang ProvinceGrant/Award Number: 2016C02058Keywordsviruses and viroidspathogen detectionornamentalsherbaceous/flowering plantsThe author(s) declare no conflict of interest.