Produção Nacional
Revisado por pares
Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018
2020; UNIVERSIDADE DE SÃO PAULO; Volume: 62; Linguagem: Inglês
10.1590/s1678-9946202062054
ISSN1678-9946
AutoresCarla Grasso Figueiredo, Adriana Luchs, Edison Luíz Durigon, Danielle Bruna Leal Oliveira, Vanessa Barbosa da Silveira, Ralyria Mello, Ana Maria Sardinha Afonso, Maria Isabel de Oliveira,
Tópico(s)Neonatal Health and Biochemistry
ResumoHuman cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positive-HCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections.
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