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Equipment contact surfaces as sources of Staphylococcus carrying enterotoxin-encoding genes in goat milk dairy plants

2020; Elsevier BV; Volume: 111; Linguagem: Inglês

10.1016/j.idairyj.2020.104827

1879-0143

Candice Maria Cardoso Gomes de Leon, F. G. C. de Sousa, Mauro de Mesquita Souza Saraiva, Patrícia Emília Naves Givisiez, Núbia Michelle Vieira da Silva, Rafael Felipe da Costa Vieira, Celso José Bruno de Oliveira,

Microbial Inactivation Methods

Potential contamination sources and the enterotoxigenic profiling of Staphylococcus isolates were investigated in four goat dairy plants. Samples (n = 160) were collected from different processing points in five samplings. Staphylococcus spp. isolates were confirmed by polymerase chain reaction and genotyped using repetitive extragenic palindromic PCR (Rep-PCR). Classical (sea, seb, sec, sed) and novel (seg, seh, sei) staphylococcal enterotoxin-encoding genes were detected by PCR. seb, sec, see and sei were identified in isolates originated from raw and pasteurised milk, pre- and post-pasteurisation tanks and wall surfaces. SE-harbouring isolates were mostly identified as non-aureus staphylococci. Genotypic profiles and contamination levels indicate that the packaging machine is a critical point for staphylococcal contamination. Equipment surfaces are important contamination sources to pasteurised milk considering the indistinguishable genotypic patterns of isolates from pre- and post-pasteurisation points. Cleaning and disinfection of milk contact surfaces should be revisited to warrant quality and safety of goat milk.