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Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein.

1987; Springer Nature; Volume: 6; Issue: 13 Linguagem: Inglês

10.1002/j.1460-2075.1987.tb02760.x

1460-2075

Walter Witke, Wolfgang Nellen, Angelika A. Noegel,

Advanced Fluorescence Microscopy Techniques

Research Article20 December 1987free access Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein. W. Witke W. Witke Max-Planck-Institut für Biochemie, Martinsried, FRG. Search for more papers by this author W. Nellen W. Nellen Max-Planck-Institut für Biochemie, Martinsried, FRG. Search for more papers by this author A. Noegel A. Noegel Max-Planck-Institut für Biochemie, Martinsried, FRG. Search for more papers by this author W. Witke W. Witke Max-Planck-Institut für Biochemie, Martinsried, FRG. Search for more papers by this author W. Nellen W. Nellen Max-Planck-Institut für Biochemie, Martinsried, FRG. Search for more papers by this author A. Noegel A. Noegel Max-Planck-Institut für Biochemie, Martinsried, FRG. Search for more papers by this author Author Information W. Witke1, W. Nellen1 and A. Noegel1 1Max-Planck-Institut für Biochemie, Martinsried, FRG. The EMBO Journal (1987)6:4143-4148https://doi.org/10.1002/j.1460-2075.1987.tb02760.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3′ portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene. Previous ArticleNext Article Volume 6Issue 131 December 1987In this issue RelatedDetailsLoading ...