Portal de Periódicos da CAPES

Immunocytochemical Demonstration of Taurine

1987; Springer Nature; Linguagem: Inglês

10.1007/978-1-4899-0405-8_29

2214-8019

Steinar Madsen, Ole Petter Ottersen, Jon Storm‐Mathisen,

Biochemical effects in animals

The exact localization of a compound in normal tissue and its redistribution in neurological diseases and disease models can give important clues as to the functional role of the compound in question. In the case of taurine (2-aminoethanesulfonic acid), so far only biochemical methods have been available for its localization. Such methods will necessarily have a limited anatomical resolution and may easily fail to detect important pathological alterations in the cellular and subcellular distribution of taurine. A much better anatomical resolution is provided by the immunocytochemical technique, which has recently been adapted to amino acids (24). Antisera against conjugated amino acids have been employed to map the light- and electronmicroscopic localization of GABA, glutamate, glutamine, asparatate, and glycine in normal nerve tissue (1,2,6,9,10,17,18,19,22,24), and have also been used to study the redistribution of transmitter amino acids in experimental models of epilepsy (14).