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BIBTEX RIS

Buccal micronucleus cytome assay: Inter-laboratory scoring exercise and micronucleus and nuclear abnormalities frequencies in different populations from Brazil

2020; Elsevier BV; Volume: 333; Linguagem: Inglês

10.1016/j.toxlet.2020.08.011

1879-3169

Paula Rohr, Gabrieli Flesch da Silva, Verônica Elisa Pimenta Vicentini, Igor Vivian de Almeida, Raquel Alves dos Santos, Catarina Satie Takahashi, Mirian Oliveira Goulart, Glenda Nicioli da Silva, Luiza Barbosa de Oliveira, César Koppe Grisólia, Tathyana Benetis Piau, Carmen Lucia Bassi Branco, Érica de Melo Reis, Marcos Felipe de Oliveira Galvão, Sílvia Regina Batistuzzo de Medeiros, Magaly Sales Monteiro, Reynaldo Assis de Vasconcelos Lopes, Sabrina Fuziger Inácio Brandão, Nelson Jorge Carvalho Batista, Márcia Fernanda Correia Jardim Paz, Juliana da Silva,

Genetically Modified Organisms Research

The Buccal Micronucleus Cytome Assay (BMCyt) has become an important biomonitoring tool for assessing cytogenetic damage in many studied populations. Each laboratory applies protocols that vary according to the method of collecting and preparing samples. Besides, Brazil is a country of great territorial extensions that received immigrants from various parts of the world with different genetic backgrounds. Therefore, the present study aimed to evaluate the inter-laboratory variation in scoring the same set of slides using the more comprehensive scoring criteria, to standardize the BMCyt protocol, to observe the basal alterations in populations of different Brazilian regions and to compare it with other places around the world. Our results showed that a valuable number of laboratories participated, ten laboratories from different regions of the country, for the validation of the BMCyt in human biomonitoring studies, resulting in the 804 healthy individuals. This was possible because we observed: a range of measures needs to be considered, such as the baseline frequency of DNA damage and cell death in non-exposed individuals; age when grouped showed an influence on DNA damage, although when evaluated by group we did not see an influence; association between smoking habit and all endpoints of the BMCyt (except karyolytic cells) was evident; the basal MN frequency, in the majority of groups, follows those around the world; and the BMCyt was confirmed as a good health status biomarker. We emphasize the need for constant discussions on the parameters of cell death due to greater difficulty among the analyzers.