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Co-transfection of normal NIH/3T3 DNA and retroval LTR sequences: a novel strategy for the detection of potential c-onc genes.

1984; Springer Nature; Volume: 3; Issue: 5 Linguagem: Inglês

10.1002/j.1460-2075.1984.tb01939.x

1460-2075

Rolf Müller, Dagmar Müller,

interferon and immune responses

Research Article1 May 1984free access Co-transfection of normal NIH/3T3 DNA and retroval LTR sequences: a novel strategy for the detection of potential c-onc genes. R. Müller R. Müller Search for more papers by this author D. Müller D. Müller Search for more papers by this author R. Müller R. Müller Search for more papers by this author D. Müller D. Müller Search for more papers by this author Author Information R. Müller and D. Müller The EMBO Journal (1984)3:1121-1127https://doi.org/10.1002/j.1460-2075.1984.tb01939.x Correction(s) for this article Co-transfection of normal NIH/3T3 DNA and retroviral LTR sequences: a novel strategy for the detection of potential c-onc genes01 June 1984 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Morphologically transformed, tumorigenic cell lines were obtained after co-transfecting normal NIH/3T3 DNA and cloned 3′-long terminal repeat sequences of Moloney leukemia virus (Mo-LTR) onto NIH/3T3 recipient cells. In four such cell lines the malignant phenotype was found to be associated with single and specific Mo-LTR integration sites that were retained after serial passages through NIH/3T3 and rat 208F cells, indicating that Mo-LTR sequences are linked to the activated oncogenes. In one of these clones the activated transforming gene was identified as c-raf, the cellular homologue of a recently described retroviral oncogene. This finding not only demonstrates that the mouse c-raf gene can be activated to exhibit an oncogenic potential but also that the approach chosen in this study is suitable for the detection of potential c-onc genes. In contrast to this clone, the activated transforming genes in other cell lines appear to be different from 19 previously isolated v-onc and c-onc genes. These results demonstrate the potential of the established transformation system for the detection and isolation of previously unidentified c-onc genes. Previous ArticleNext Article Volume 3Issue 51 May 1984In this issue RelatedDetailsLoading ...