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Biological control of mites by xerophile Eurotium species isolated from the surface of dry cured ham and dry beef cecina

2020; Oxford University Press; Volume: 130; Issue: 3 Linguagem: Inglês

10.1111/jam.14839

1365-2672

José F. Ortiz-Lemus, Sonia Campoy, Juan F. Martı́n,

Plant and fungal interactions

Journal of Applied MicrobiologyVolume 130, Issue 3 p. 665-676 Original Article Biological control of mites by xerophile Eurotium species isolated from the surface of dry cured ham and dry beef cecina J.F. Ortiz-Lemus, Área de Microbiología, Departmento de Biología Molecular, Universidad de León, León, Spain Instituto de Biotecnología de León, INBIOTEC, León, Spain Departamento de Microbiología, Universidad de Pamplona, Pamplona, ColombiaSearch for more papers by this authorS. Campoy, Área de Microbiología, Departmento de Biología Molecular, Universidad de León, León, Spain Instituto de Biotecnología de León, INBIOTEC, León, SpainSearch for more papers by this authorJ.F. Martín, Corresponding Author jf.martin@unileon.es orcid.org/0000-0003-0156-2059 Área de Microbiología, Departmento de Biología Molecular, Universidad de León, León, Spain Instituto de Biotecnología de León, INBIOTEC, León, Spain Correspondence Juan F. Martín, Área de Microbiología, Departmento de Biología Molecular, Universidad de León, 24071 León, Spain. E-mail: jf.martin@unileon.esSearch for more papers by this author J.F. Ortiz-Lemus, Área de Microbiología, Departmento de Biología Molecular, Universidad de León, León, Spain Instituto de Biotecnología de León, INBIOTEC, León, Spain Departamento de Microbiología, Universidad de Pamplona, Pamplona, ColombiaSearch for more papers by this authorS. Campoy, Área de Microbiología, Departmento de Biología Molecular, Universidad de León, León, Spain Instituto de Biotecnología de León, INBIOTEC, León, SpainSearch for more papers by this authorJ.F. Martín, Corresponding Author jf.martin@unileon.es orcid.org/0000-0003-0156-2059 Área de Microbiología, Departmento de Biología Molecular, Universidad de León, León, Spain Instituto de Biotecnología de León, INBIOTEC, León, Spain Correspondence Juan F. Martín, Área de Microbiología, Departmento de Biología Molecular, Universidad de León, 24071 León, Spain. E-mail: jf.martin@unileon.esSearch for more papers by this author First published: 31 August 2020 https://doi.org/10.1111/jam.14839Citations: 2Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract Some meat dry products, including dry cured ham and dry beef cecina, are cured in cellars at moderately cold temperature allowing the growth of a lawn of fungi on their surface. During the curing process, frequently these products became contaminated with fungivore mites of the Acaridae family that feed on fungal mycelium and spores. Aims The aim of this article is to study the possible biological control of mites by fungi that form part of the normal microbiota of these meat products. Methods and Results Some yellow/orange pigmented fungi growing on the ham surface decreased the proliferation of mites; therefore, we isolated from ham and cecina xerophilic yellow/orange coloured fungal strains that were identified as members of the genus Eurotium (recently reclassified as Aspergillus section Aspergillus). Using molecular genetic tools, we have identified 158 strains as Eurotium rubrum (Aspergillus ruber), Eurotium repens (Aspergillus pseudoglaucus) and Eurotium chevalieri (Aspergillus chevalieri). Two strains, E. rubrum C47 and E. rubrum C49, showed strong miticidal activity. The toxic compound(s) are associated with the formation of cleistothecia. In synchronized mite development experiments, we observed that all stages of the mite lifecycle were inhibited by the E. rubrum C47 strain. In addition, we searched for miticidal activity in 13 culture collection Eurotium strains isolated from different habitats, and found that only one, Eurotium cristatum NRRL 4222 (Aspergillus cristatus) has a strong miticidal activity. Conclusions These fungal strains have proliferated on the surface of ham and cecina for decades, and possibly have acquired miticidal activity as a resistance mechanism against fungivores. Significance and Impact of the Study Biological control of infecting mites by favouring growth of E. rubrun C47, in place of the normal mixed population of Aspergillus and Penicillium, is an attractive approach to control mite infestations. Citing Literature Supporting Information Filename Description jam14839-sup-0001-TableS1.docxWord document, 15 KB Table S1. Culture collection fungi used in this study as control for the identification of the fungal isolates Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume130, Issue3March 2021Pages 665-676 RelatedInformation