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Potential antileptospiral constituents from Phyllanthus amarus

2020; SAGE Publishing; Volume: 16; Issue: 70 Linguagem: Inglês

10.4103/pm.pm_10_20

0976-4062

S Chandan, S. Umesha, KShiva Prasad, V. Balamurugan, S. Chandrashekar, SR Santosh Kumar, Ramith Ramu, PrithviS Shirahatti, Asad Syed, Abdallah M. Elgorban,

Phytochemistry and Bioactivity Studies

Pharmacognosy Magazine,2020,16,70,371-378.DOI:10.4103/pm.pm_10_20Published:August 2020Type:Original ArticleAuthors:S Chandan, S Umesha, K Shiva Prasad, V Balamurugan, S Chandrashekar, SR Santosh Kumar, Ramith Ramu, Prithvi S Shirahatti, Asad Syed, and Abdallah M Elgorban Author(s) affiliations:S Chandan1, S Umesha2, K Shiva Prasad3, V Balamurugan4, S Chandrashekar5, SR Santosh Kumar6, Ramith Ramu1, Prithvi S Shirahatti7, Asad Syed8, Abdallah M Elgorban8 1 Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India 2 Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India 3 Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka, India 4 Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka, India 5 Department of Biotechnology, Davangere University, Shivagangothri, Davangere, Karnataka, India 6 Department of Food Technology, Davangere University, Shivagangothri, Davangere, Karnataka, India 7 Department of Biotechnology, Teresian College, Siddhartha Nagara, Mysuru, Karnataka, India 8 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia Abstract:Background: Phyllanthus amarus (PA) is a well-known herb for its medicinal properties and widely used worldwide. PA has a significant role in Indian Ayurveda system of medicine for treating various ailments such as gonorrhea, menorrhagia, and other genital infections. Objectives: The aim of the study is to investigate antileptospiral activity and isolate the potential antileptospiral constituents of the methanol extract of PA (MPA). Materials and Methods: The primary pharmacological tests for leptospirosis were investigated by test tube dilution technique and microdilution technique. To examine the morphogenesis of experimental leptospirosis by morphologic and histological methods, albino mice were inoculated intraperitoneally with Leptospira interrogans sero group Icterohaemorrhagiae strains. Results: The activity-guided repeated fractionation for MPA through silica gel column chromatography yielded three compounds that exhibited antioxidant and in vitro, in vivo, and in silico antileptospiral activities. Based on diverse physicochemical and spectroscopic analyses (viz.,13C NMR,1H NMR, ultraviolet [UV], IR, and mass spectroscopy), the potential constituents were elucidated as 5-(3-(3,4-dimethoxybenzyl)-4-methoxy-2-(methoxymethyl)butyl)-4,7-dimethoxybenzo[d][1,3]dioxole(C1),1-(3-(3,4-dimethoxybenzyl)-4-methoxy-2-(methoxymethyl)butyl)-2,3,4,5tetramethoxybenzene(C2), and 4-(3-(3,4dimethoxybenzyl)-4-methoxy-2-(methoxymethyl)butyl)-3,6-dimethoxybenzene-1,2-diol (C3). The histopathological examinations of both kidney and liver showed promising activity with C3 at 75 and 100 μg/mL, respectively. Conclusion: The in vitro , in vivo, and in silico studies revealed that benzo methoxy class of compounds has great potential as antileptospiral agents. Keywords:Antileptospiral activity, antioxidant, Bioactive compounds, in silico, Leptospira, Phyllanthus amarusView:PDF (2.21 MB)