SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification
2020; National Academy of Sciences; Volume: 117; Issue: 39 Linguagem: Inglês
10.1073/pnas.2011221117
ISSN1091-6490
AutoresBrian Rabe, Constance L. Cepko,
Tópico(s)Advanced Chemical Sensor Technologies
ResumoSignificance This work describes the optimization of a sample preparation and detection pipeline for a SARS-CoV-2 diagnostic test that is rapid and does not require specialized equipment. This pipeline consists of viral inactivation, rendering samples safer to work with, followed by a sensitive 30-min isothermal detection reaction with a color-based red to yellow readout. Sensitivity can be further improved using a simple and inexpensive purification protocol. This pipeline can help address the shortage of testing capacity and can be run in a variety of settings.
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