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SARS-CoV-2 envelope protein topology in eukaryotic membranes

2020; Royal Society; Volume: 10; Issue: 9 Linguagem: Inglês

10.1098/rsob.200209

2046-2441

Gerard Duart, Ma Jesús García-Murria, Brayan Grau, José M. Acosta-Cáceres, Luis Martínez‐Gil, Ismael Mingarro,

Viral gastroenteritis research and epidemiology

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.