First Report of Colletotrichum siamense and C. gloeosporioides Causing Anthracnose of Citrus spp. in Mexico
2020; American Phytopathological Society; Volume: 105; Issue: 2 Linguagem: Inglês
10.1094/pdis-08-20-1743-pdn
ISSN1943-7692
AutoresJ. L. Pérez-Mora, Guadalupe Arlene Mora‐Romero, Hugo Beltrán‐Peña, Elizabeth García‐León, Nelson Bernardi Lima, M. Camacho-Tapía, Juan Manuel Tovar‐Pedraza,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 105, No. 2First Report of Colletotrichum siamense and C. gloeosporioides Causing Anthracnose of Citrus spp. in Mexico PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum siamense and C. gloeosporioides Causing Anthracnose of Citrus spp. in MexicoJ. L. Pérez-Mora, G. A. Mora-Romero, H. Beltrán-Peña, E. García-León, N. B. Lima, M. Camacho-Tapia, and J. M. Tovar-PedrazaJ. L. Pérez-MoraUniversidad Autónoma de Occidente, Unidad Regional Los Mochis, Departamento de Ciencias Naturales y Exactas, Los Mochis, 81223, Sinaloa, Mexico, G. A. Mora-RomeroUniversidad Autónoma de Occidente, Unidad Regional Los Mochis, Departamento de Ciencias Naturales y Exactas, Los Mochis, 81223, Sinaloa, Mexico, H. Beltrán-PeñaUniversidad Autónoma de Occidente, Unidad Regional Los Mochis, Departamento de Ciencias Naturales y Exactas, Los Mochis, 81223, Sinaloa, Mexico, E. García-LeónInstituto Nacional de Investigaciones Agrícolas, Forestales y Pecuarias, Campo Experimental Valle del Fuerte, Guasave, 81110, Sinaloa, Mexico, N. B. Limahttp://orcid.org/0000-0002-6145-5119Instituto de Patología Vegetal (CIAP-INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET, Córdoba, X5020, Argentina, M. Camacho-TapiaUniversidad Autónoma Chapingo, Laboratorio Nacional de Investigación y Servicio Agroalimentario y Forestal, Texcoco, 56230, Estado de México, Mexico, and J. M. Tovar-Pedraza†Corresponding author: J. M. Tovar-Pedraza; E-mail Address: juan.tovar@ciad.mxhttp://orcid.org/0000-0002-8560-959XCentro de Investigación en Alimentación y Desarrollo, Coordinación Regional Culiacán, Laboratorio de Fitopatología, Culiacán, 80110, Sinaloa, Mexico AffiliationsAuthors and Affiliations J. L. Pérez-Mora1 G. A. Mora-Romero1 H. Beltrán-Peña1 E. García-León2 N. B. Lima3 M. Camacho-Tapia4 J. M. Tovar-Pedraza5 † 1Universidad Autónoma de Occidente, Unidad Regional Los Mochis, Departamento de Ciencias Naturales y Exactas, Los Mochis, 81223, Sinaloa, Mexico 2Instituto Nacional de Investigaciones Agrícolas, Forestales y Pecuarias, Campo Experimental Valle del Fuerte, Guasave, 81110, Sinaloa, Mexico 3Instituto de Patología Vegetal (CIAP-INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET, Córdoba, X5020, Argentina 4Universidad Autónoma Chapingo, Laboratorio Nacional de Investigación y Servicio Agroalimentario y Forestal, Texcoco, 56230, Estado de México, Mexico 5Centro de Investigación en Alimentación y Desarrollo, Coordinación Regional Culiacán, Laboratorio de Fitopatología, Culiacán, 80110, Sinaloa, Mexico Published Online:15 Dec 2020https://doi.org/10.1094/PDIS-08-20-1743-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleCitrus anthracnose, caused by Colletotrichum spp., is a major disease in many citrus-growing regions of the world. During the spring of 2019, symptoms of petal necrosis and necrotic lesions on fruits were detected on Mexican lime (Citrus aurantifolia), sweet orange (Citrus sinensis), and grapefruit (Citrus paradisi) trees in three commercial orchards distributed in northern Sinaloa (El Fuerte and Ahome municipalities), Mexico. Colletotrichum-like colonies were consistently isolated on potato dextrose agar (PDA) medium from symptomatic petals and fruits, and 30 monoconidial isolates (10 per orchard) were obtained. Five isolates were selected as representative for morphological characterization, multilocus phylogenetic analysis, and pathogenicity tests. The isolates were designated as FAVF355 to FAVF359 and were deposited in the Culture Collection of Phytopathogenic Fungi of the Faculty of Agronomy of El Fuerte Valley at the Autonomous University of Sinaloa (Mexico). Colonies grown on PDA at 25°C were cottony, dense, with grayish white aerial mycelium and with pink conidial masses. Conidia (n = 100) were cylindrical, hyaline, aseptate, 13.7 to 18.8 × 4.3 to 5.8 μm, with both ends rounded. Based on morphological features, the five isolates were tentatively identified in the Colletotrichum gloeosporioides species complex (Weir et al. 2012). For molecular identification, total DNA was extracted, and the internal transcribed spacer (ITS) region (White et al. 1990) and partial sequences of actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β-tubulin (TUB2) genes were amplified by polymerase chain reaction (Weir et al. 2012) and sequenced. A phylogenetic tree based on Bayesian inference for species belonging to the C. gloeosporioides species complex was constructed. The multilocus phylogenetic analysis distinguished the isolates FAVF355 to FAVF357 as C. gloeosporioides sensu stricto and the isolates FAVF358 to FAVF359 as C. siamense. The sequences were deposited in GenBank (ITS, MT850050 to MT850054; ACT, MT834528 to MT834532; GAPDH, MT855979 to MT855982; TUB2, MT834533 to MT834536). Pathogenicity of the five isolates was verified on healthy fruits of their original host species. Five fruits per isolate were inoculated using the colonized agar plug method. Fruits were wounded with a sterile toothpick, and mycelial plugs (5 mm in diameter) removed from the margin of a 6-day-old culture were placed onto three wound sites in each fruit. Noncolonized agar plugs were placed on the wounds of 10 fruits used as the control. The fruits were kept in a moist chamber at 25°C for 8 days. The experiment was repeated twice. All inoculated fruits developed circular and necrotic lesions 6 days after inoculation, whereas the control fruits remained symptomless. The fungi were consistently reisolated from the diseased fruits and were morphologically identical to those originally inoculated, fulfilling Koch's postulates. To date, only C. gloeosporioides sensu lato and C. acutatum sensu lato have been associated with sweet orange and Mexican lime in Mexico (Farr and Rossman 2020), whereas C. gloeosporioides sensu stricto has been recently recorded in a different area (Iguala, Guerrero) of Mexico (Cruz-Lagunas et al. 2020). To our knowledge, this is the first report of C. gloeosporioides sensu stricto causing anthracnose on sweet orange, and of C. siamense on Mexican lime in Mexico, as well as C. gloeosporioides sensu stricto causing disease on grapefruit in Sinaloa, Mexico.The author(s) declare no conflict of interest.References:Cruz-Lagunas, B., et al. 2020. Australas. Plant Dis. Notes 15:31. Crossref, Google ScholarFarr, D. F., and Rossman, A. Y. 2020. Fungal Databases, Syst. Mycol. Microbiol. Lab., ARS, USDA. Retrieved July 15, 2020, from https://nt.ars-grin.gov/fungaldatabases/. Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.Funding: This research was supported by the National Council of Science and Technology of Mexico (CONACYT), project INFRA–2019–302117.DetailsFiguresLiterature CitedRelated Vol. 105, No. 2 February 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionDeformation of a bud in blackcurrant after infestation with the mite Cecidophyopsis ribis, Acari: Eriophyidae (Špak et al.). Photo credit: J. Špak. Symptoms of sugar beet rubbery taproot disease (Ćurčić et al.). Photo credit: B. Duduk. Metrics Downloaded 1,874 times Article History Issue Date: 24 Feb 2021Published: 15 Dec 2020First Look: 10 Sep 2020Accepted: 9 Sep 2020 Pages: 496-496 Information© 2021 The American Phytopathological SocietyFundingNational Council of Science and Technology of Mexico (CONACYT)Grant/Award Number: INFRA-2019-302117KeywordsfungiphylogenymorphologypathogenicityThe author(s) declare no conflict of interest.PDF downloadCited ByDistribution and characterization of Colletotrichum species associated with Citrus anthracnose in eastern Mediterranean region of Turkey14 February 2022 | European Journal of Plant Pathology, Vol. 163, No. 1An Eleven-Year Survey on Field Disease Susceptibility of Citrus Accessions to Colletotrichum and Alternaria Species10 June 2021 | Agriculture, Vol. 11, No. 6Colletotrichum Species Causing Anthracnose of Citrus in Australia12 January 2021 | Journal of Fungi, Vol. 7, No. 1
Referência(s)