First Report of Fusarium falciforme (FSSC 3+4) Causing Wilt Disease of Phaseolus vulgaris in Mexico
2020; American Phytopathological Society; Volume: 105; Issue: 3 Linguagem: Inglês
10.1094/pdis-06-20-1160-pdn
ISSN1943-7692
AutoresJosé Francisco Díaz-Nájera, Sergio Ayvar-Serna, Antonio Mena-Bahena, Emiliano Baranda-Cruz, Mateo Vargas, Omar G. Alvarado-Gómez, Dionicio Fuentes‐Aragón,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 105, No. 3First Report of Fusarium falciforme (FSSC 3+4) Causing Wilt Disease of Phaseolus vulgaris in Mexico PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium falciforme (FSSC 3+4) Causing Wilt Disease of Phaseolus vulgaris in MexicoJosé F. Díaz-Nájera, Sergio Ayvar-Serna, Antonio Mena-Bahena, Emiliano Baranda-Cruz, Mateo Vargas-Hernández, Omar G. Alvarado-Gómez, and Dionicio Fuentes-AragónJosé F. Díaz-NájeraDepartamento de Fitotecnia, Centro de Estudios Profesionales, Colegio Superior Agropecuario del Estado de Guerrero, Cocula, Guerrero, Mexico, Sergio Ayvar-SernaDepartamento de Fitotecnia, Centro de Estudios Profesionales, Colegio Superior Agropecuario del Estado de Guerrero, Cocula, Guerrero, Mexico, Antonio Mena-BahenaDepartamento de Fitotecnia, Centro de Estudios Profesionales, Colegio Superior Agropecuario del Estado de Guerrero, Cocula, Guerrero, Mexico, Emiliano Baranda-CruzDepartamento de Fitotecnia, Centro de Estudios Profesionales, Colegio Superior Agropecuario del Estado de Guerrero, Cocula, Guerrero, Mexico, Mateo Vargas-HernándezDepartamento de Parasitología Agrícola, Universidad Autónoma Chapingo, Texcoco, Estado de México, Mexico, Omar G. Alvarado-GómezFacultad de Agronomía, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, Mexico, and Dionicio Fuentes-Aragón†Corresponding author: D. Fuentes-Aragón; E-mail Address: fuentes.dionicio@colpos.mxhttp://orcid.org/0000-0002-2714-2477Posgrado en Fitosanidad, Colegio de Postgraduados, Campus Montecillo, Texcoco, Estado de México, Mexico AffiliationsAuthors and Affiliations José F. Díaz-Nájera1 Sergio Ayvar-Serna1 Antonio Mena-Bahena1 Emiliano Baranda-Cruz1 Mateo Vargas-Hernández2 Omar G. Alvarado-Gómez3 Dionicio Fuentes-Aragón4 † 1Departamento de Fitotecnia, Centro de Estudios Profesionales, Colegio Superior Agropecuario del Estado de Guerrero, Cocula, Guerrero, Mexico 2Departamento de Parasitología Agrícola, Universidad Autónoma Chapingo, Texcoco, Estado de México, Mexico 3Facultad de Agronomía, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, Mexico 4Posgrado en Fitosanidad, Colegio de Postgraduados, Campus Montecillo, Texcoco, Estado de México, Mexico Published Online:25 Jan 2021https://doi.org/10.1094/PDIS-06-20-1160-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleBean (Phaseolus vulgaris) is the second most important crop in Mexico after corn due to the high consumption of beans in all regions of the country. In the winter (January 2016), bean plants showing wilting, root discoloration, and necrosis were observed, with an incidence of approximately 30% in different fields (<1 ha) in Tecoanapa, Guerrero State, Mexico. Symptomatic fine roots (<2 mm) were cut into 0.5-cm-long pieces, washed with tap water, surface disinfected with 1.5% NaOCl for 3 min, and rinsed with sterile distilled water. Thirty-five pieces were placed on potato dextrose agar (PDA, Difco) and incubated at 25°C for 7 days. Then, single-spore isolates were obtained. Colonies on PDA showed abundant white aerial mycelium and a growth rate of 4.5 mm/day, and in reverse, colonies were white/pink with a brown center. Microconidia were cylindrical to ellipsoid, aseptate, hyaline and 7.8 to (6.0) to 4.7 × 2.7 to (2.1) to 1.6 µm. On carnation leaf agar, macroconidia were 37.8 to (29.4) to 23.5 × 4.1 to (3.5) to 2.6 µm, hyaline, falcate, with slightly curved apexes, and three to five septa. Chlamydospores were round, intercalary, hyaline, single or in chains (Booth 1971). A representative strain (CSAEGRO-AyDi-Ef) was analyzed by polymerase chain reaction, and the translation elongation factor 1-alpha (tef1) gene (GenBank accession no. MK945757) was sequenced using the EF-1/EF-2 primers (O'Donnell 2000). Fusarium-ID (Geiser et al. 2004) analysis showed 100% similarity with the Fusarium solani species complex (FSSC 3+4) strain NRRL28562. In addition, Bayesian phylogenetic analysis placed this strain in the Fusarium falciforme clade. A pathogenicity test was performed by immersing healthy plant roots (cv. Negro Jamapa) in 200 ml of a conidial suspension (5 × 107 conidia/ml) for 10 min and then transplanting the plants into pots. Control plants were immersed in sterile distilled water. Similar symptoms as those in the field were observed at 10 days after inoculation, and the controls were healthy. The fungus was reisolated from infected plants and showed the same morphology and tef1 sequence as the original isolate, fulfilling Koch's postulates. Recently, F. falciforme was reported to cause wilting of P. vulgaris in Cuba (Duarte et al. 2019); however, this is the first report of F. falciforme (FSSC 3+4) causing wilt disease of P. vulgaris in Mexico. This species was previously reported in Mexico affecting onion (Tirado-Ramírez et al. 2018), papaya, tomato (Vega-Gutiérrez et al. 2019a, 2019b), and maize (Douriet-Angulo et al. 2019), suggesting an ample host range in the country.The author(s) declare no conflict of interest.References:Booth, C. 1971. The Genus Fusarium. Commonwealth Mycological Institute, Kew, U.K. Google ScholarDouriet-Angulo, A., et al. 2019. Plant Dis. 103:2951. https://doi.org/10.1094/PDIS-05-19-1055-PDN Link, Google ScholarDuarte, Y., et al. 2019. New Dis. Rep. 40:9. https://doi.org/10.5197/j.2044-0588.2019.040.009 Crossref, Google ScholarGeiser, D. M., et al. 2004. Eur. J. Plant Pathol. 110:473. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0 Crossref, ISI, Google ScholarO'Donnell, K. 2000. Mycologia 92:919. https://doi.org/10.1080/00275514.2000.12061237 Crossref, ISI, Google ScholarTirado-Ramírez, M. A., et al. 2018. Plant Dis. 102:2646. https://doi.org/10.1094/PDIS-05-18-0757-PDN Link, ISI, Google ScholarVega-Gutiérrez, T. A., et al. 2019a. Plant Dis. 103:2681. https://doi.org/10.1094/PDIS-05-19-0917-PDN Link, Google ScholarVega-Gutiérrez, T. A., et al. 2019b. Plant Dis. 103:157. https://doi.org/10.1094/PDIS-06-18-1001-PDN Link, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 105, No. 3 March 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionMaize ear showing scattered moldy or "starburst" symptoms, typical of Fusarium verticillioides (A. F. Logrieco et al.). Photo credit: G. Munkvold. Bright yellow vein banding, rings, and lines associated with alfalfa mosaic virus infection in chayote (Sechium edule (Jacq.) Sw.) (G. Parrella et al.). Photo credit: G. Parrella. Metrics Downloaded 820 times Article History Issue Date: 26 Mar 2021Published: 25 Jan 2021First Look: 11 Sep 2020Accepted: 8 Sep 2020 Pages: 710-710 Information© 2021 The American Phytopathological SocietyKeywordsFusariumPhaseolus vulgarisfungiThe author(s) declare no conflict of interest.Cited ByGuava decline: updating its etiology from 'Fusarium solani' to Neocosmospora falciformis19 November 2020 | European Journal of Plant Pathology, Vol. 159, No. 2
Referência(s)