Produção Nacional
Revisado por pares
Antinociceptive activity of 3β-6β-16β-trihydroxylup-20 (29)-ene triterpene isolated from Combretum leprosum leaves in adult zebrafish (Danio rerio)
2020; Elsevier BV; Volume: 533; Issue: 3 Linguagem: Inglês
10.1016/j.bbrc.2020.07.107
ISSN1090-2104
AutoresFrancisca Crislândia Oliveira Silva, Jane Eire Silva Alencar de Menezes, Maria Kueirislene Amâncio Ferreira, Antônio Wlisses da Silva, Carlos Leone Alves Holanda, Joyce dos Reis Lima, Adriana Rolim Campos, Francisco Flávio Vasconcelos Evaristo, Edson Holanda Teixeira, Francisco Ernani Alves Magalhães, Paulo Nogueira Bandeira, Hélcio Silva dos Santos,
Tópico(s)Pain Mechanisms and Treatments
ResumoDrugs used to treat pain are associated with adverse effects, increasing the search for new drugs as an alternative treatment for pain. Therefore, we evaluated the antinociceptive behavior and possible neuromodulation mechanisms of triterpene 3β, 6β, 16β-trihydroxylup-20(29)-ene (CLF-1) isolated from Combretum leprosum leaves in zebrafish. Zebrafish (n = 6/group) were pretreated with CLF-1 (0.1 or 0.3 or 1.0 mg/mL; i.p.) and underwent nociception behavior tests. The antinociceptive effect of CFL-1 was tested for modulation by opioid (naloxone), nitrergic (L-NAME), nitric oxide and guanylate cyclase synthesis inhibitor (methylene blue), NMDA (Ketamine), TRPV1 (ruthenium red), TRPA1 (camphor), or ASIC (amiloride) antagonists. The corneal antinociceptive effect of CFL-1 was tested for modulation by TRPV1 (capsazepine). The effect of CFL-1 on zebrafish locomotor behavior was evaluated with the open field test. The acute toxicity study was conducted. CLF-1 reduced nociceptive behavior and corneal in zebrafish without mortalities and without altering the animals' locomotion. Thus, CFL-1 presenting pharmacological potential for the treatment of acute pain and corneal pain, and this effect is modulated by the opioids, nitrergic system, NMDA receptors and TRP and ASIC channels.
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