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Structural characterization, antifungal and cytotoxic profiles of quaternized heteropolysaccharide from Anadenanthera colubrina

2020; Elsevier BV; Volume: 165; Linguagem: Inglês

10.1016/j.ijbiomac.2020.09.087

1879-0003

Fábio de Oliveira Silva Ribeiro, Gisele Santos de Araújo, Maria Gabriela Araújo Mendes, Tatiane Caroline Daboit, Lucas Moreira Brito, Cláudia Pessoa, Laís Ramos Monteiro de Lima, Regina C.M. de Paula, Ruan Sousa Bastos, Jefferson Almeida Rocha, Égil de Brito Sá, Thaísa Cardoso de Oliveira, Antônia Carla de Jesus Oliveira, José Lamartine Soares‐Sobrinho, José Roberto de Souza de Almeida Leite, Alyne Rodrigues de Araújo, Durcilene Alves da Silva,

Nanoparticles: synthesis and applications

In the present work, we investigated the minimal inhibitory concentration (MIC) against fungal strains (Fonsecaea pedrosoi, Microsporum canis, Candida albicans, Cryptococcus neoformans), and cytotoxicity to normal cell lines for modified red angico gum (AG) with eterifying agent N-chloride (3-chloro-2-hydroxypropyl) trimethylammonium (CHPTAC). Quaternized ammonium groups were linked to AG backbone using N-(3-chloro-2-hydroxypropyl) trimethylammonium chloride. The chemical features of the quaternized gum derivatives (QAG) were analyzed by: FTIR, elemental analysis, Zeta potential and gel permeation chromatography. The angico quaternizated gum presented a degree of substitution (DS) of 0.22 and Zeta potential of +36.43. For the antifungal test, it was observed that unmodified gum did not inhibit fungal growth. While, QAG inhibited the growth of most fungi used in this study. By AFM technique QAG interacted with the fungal surface, altering wall roughness significantly. The probable affinity of fragments of the QAG structure for the fungal enzyme 5I33 (Adenylosuccinate synthetase) has been shown by molecular docking. Low cytotoxicity was observed for polymers (unmodified gum and QAG). The results demonstrate that the quaternized polymer of AG presented in this study is a quite promising biomaterial for biotechnological applications.