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Reemergent Cases of COVID-19 — Xinjiang Uygur Autonomous Region, China, July 16, 2020

2020; Volume: 2; Issue: 39 Linguagem: Inglês

10.46234/ccdcw2020.206

2097-3101

Cao Chen, Hemuti Ma, Zhiyuan Jia, Xiang Zhao, Dayan Wang, Jun Zhao, Zhenguo Gao, Peipei Liu, Yang Song, Zhixiao Chen, Yuchao Wu, Yao Meng, Guizhen Wu, Wenbo Xu, Xucheng Fan, Yong Zhang,

SARS-CoV-2 detection and testing

Before the coronavirus disease 2019 (COVID-19) outbreak on July 16, 2020, no new COVID-19 cases were reported in Urumqi City of Xinjiang for 150 consecutive days.During this outbreak, 826 confirmed COVID-19 cases have been reported in Urumqi.The phylogenetic characteristics of COVID-19 virus from clinical specimens of the initial 4 cases in the Urumqi outbreak were sequenced.The full-length genome sequencing of COVID-19 virus was performed on the Illumina MiSeq platform, and 4 full-length COVID-19 virus genome sequences were obtained from clinical specimens of the initial 4 cases in this outbreak.Compared with the reference sequence EPI_ISL_402119 (1), which was isolated from Wuhan City in Hubei Province on January 7, 2020.A total of 13 nucleotide variations were determined, among which 7 nucleotide variations (C241T, C3037T, C14408T, A23403G, G28881A, G28882A, and G28883C) were identified in each fulllength genome of the 4 Xinjiang strains, which were consistent with the characteristics of the L-lineage European branch 1/B.1.1 according to the latest classification principle (2) (Figure 1).The Xinjiang strains were different from the previous hCoV-19/Wuhan/IVDC-HB-01/2019 hCoV-19/Wuhan/ IVDC-HB-03/2019 hCoV-19/Wuhan/IVDC-HB-05/2019 (belonging to S-lineage/A) strains in Wuhan in December 2019, indicating that they were not continuous transmission of indigenous COVID-19 virus strains.Other nucleotide substitutions were also identified in these Xinjiang strains, including C12789T and G14118T substitutions in all 4 genomes of the viruses, C2197T in specimen 1, C12809T in specimen 2, and C19718T and C23481T in specimen 3.Since no Xinjiang strains contained the characteristic substitutions of Beijing Xinfadi strains (C6024T) (3) and Dalian strains (C2091T, A5128G, A8360G, C13860T, T19839C, G19999T, and C28905T) (4) and because the Beijing Xinfadi strains and Dalian Fundings: