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BIBTEX RIS

Automated identification of leukocyte subsets improves standardization of database-guided expert-supervised diagnostic orientation in acute leukemia: a EuroFlow study

2020; Elsevier BV; Volume: 34; Issue: 1 Linguagem: Inglês

10.1038/s41379-020-00677-7

1530-0285

Ludovic Lhermitte, Sylvain Barreau, Daniela Morf, Paula Fernández, Georgiana Grigore, Susana Bárrena, Maaike de Bie, Juan Flores‐Montero, Monika Brüggemann, Ester Mejstříková, Stefan Nierkens, Leire Burgos, Joana Caetano, Giuseppe Gaipa, Chiara Buracchi, Elaine Sobral da Costa, Łukasz Sędek, Tomasz Szczepański, Carmen-Mariana Aanei, Alita van der Sluijs-Gelling, Alejandro Hernández-Delgado, Rafael Fluxá, Quentin Lécrevisse, Carlos E. Pedreira, Jacques J. M. van Dongen, Alberto Órfão, Vincent H. J. van der Velden, Jacques J. M. van Dongen, W. Marieke Bitter, Brad Lubbers, C.I. Teodosio, Mihaela Zlei, Alita J. van der Sluijs‐Gelling, F. de Bie, Sandra de Bruin-Versteeg, Mirjam van der Burg, Marco W. Schilham, Vincent H. J. van der Velden, Anton W. Langerak, Jeroen te Marvelde, A.E. Bras, Joyce Schilperoord‐Vermeulen, Romana Jugooa, Kim C. Heezen, Alberto Órfão, Júlia Almeida, María‐Belén Vidriales, Juan Flores‐Montero, Martín Pérez‐Andres, Sergio Matarraz, L. Martín, Quentin Lécrevisse, J.J. Pérez-Morán, N. Puig, António Almeida, María Gomes da Silva, Tiago Faria, Monika Brüggemann, Matthias Ritgen, Monika Szczepanowski, Saskia Kohlscheen, Anna Laqua, E. Harbst, Jürgen Finke, Vahid Asnafi, Ludovic Lhermitte, Eugénie Duroyon, Jan Trka, Ondřej Hrušák, Tomáš Kalina, Ester Mejstříková, Michaela Nováková, Daniel Thürner, Veronika Kanderová, Tomasz Szczepański, Łukasz Sędek, Joanna Bulsa, Łukasz Słota, Jan Kulis, Carlos E. Pedreira, Elaine Sobral da Costa, Stefan Nierkens, Anneke de Jong, Arjan de Koning, Margarida Lima, Andres Santos, Sebastian Böttcher, Sandra Lange, R. Engelmann, Daniel Paape, C. Machka, Giuseppe Gaipa, C. Burracchi, Cristina Bugarin, Eduardo López‐Granados, Lucía del Pino Molina, Lydia Campos-Guyotat, Carmen Aanei, Jesús F. San Miguel, Bruno Paiva, Leire Burgos, N. Villamor-Casas, Laura Magnano, Jan Philippé, Carolien Bonroy, Barbara Denys, A. Willems, Pauline Breughe, Jtm de Wolf, Ana E. Sousa, S.L. Silva, Philippe Fernandez, D. Morf,

Biosensors and Analytical Detection

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant classification panel and final diagnosis. In this study, we designed and validated an algorithm for automated (database-supported) gating and identification (AGI tool) of cell subsets within samples stained with ALOT. A reference database of normal peripheral blood (PB, n = 41) and bone marrow (BM; n = 45) samples analyzed with the ALOT was constructed, and served as a reference for the AGI tool to automatically identify normal cells. Populations not unequivocally identified as normal cells were labeled as checks and were classified by an expert. Additional normal BM (n = 25) and PB (n = 43) and leukemic samples (n = 109), analyzed in parallel by experts and the AGI tool, were used to evaluate the AGI tool. Analysis of normal PB and BM samples showed low percentages of checks (<3% in PB, 0.95 for all cell types in PB and r2 > 0.75 in BM) and resulted in highly concordant classification of leukemic cells by our previously published automated database-guided expert-supervised orientation tool for immunophenotypic diagnosis and classification of acute leukemia (Compass tool). Similar data were obtained using alternative, commercially available tubes, confirming the robustness of the developed tools. The AGI tool represents an innovative step in minimizing human intervention and requirements in expertise, toward a "sample-in and result-out" approach which may result in more objective and reproducible data analysis and diagnostics. The AGI tool may improve quality of immunophenotyping in individual laboratories, since high percentages of checks in normal samples are an alert on the quality of the internal procedures.