Molecular Detection of Trypanosoma species in Cattle Slaughtered at Zango and Yantukwane Abattoirs in Zaria, Kaduna State, Nigeria
2020; African Journals OnLine; Volume: 41; Issue: 2 Linguagem: Inglês
10.4314/njpar.v41i2.7
ISSN1117-4145
AutoresIsmaliza Isa, Lidwina Salim, B Kamba,
Tópico(s)Vector-Borne Animal Diseases
ResumoAfrican animal trypanosomoses constitute the most important vector-borne cattle diseases in Sub-Saharan Africa. A total of170 blood samples were collected from cattle in Zango abattoir and Yantukwane abattoir in Zaria, Kaduna State. BloodSamples collected were examined for the presence of trypanosomes using the buffy coat technique and Giemsa thin bloodsmears which shows a single 1(0.58%; 1/170) infection using microscope. Positive and negative samples were subjected tofurther analysis using nested PCR reaction with nested primers for detection of trypanosomes, and 9 of the samples showedpositive bands after gel electrophoresis at 998bp (lane 1-9) indicating the presence of Trypanosoma theileri. Percentage, Chisquareand Odds ratio were used to analyse the data. A total of 9(5.29%; 9/170) animals were found infected with the parasiteout of which 4(4.65%; 4/85) were found in Zango abattoir, while 5 (5.95%) were found in Yantukwane abattoir. The resultswere not statistically (P>0.05) location dependent. The infection in male (6.45%) cattle was higher than the female (4.63%)with no infection in young animals. No infection was observed in Sokoto Gudali breed, while White Fulani breed had 5.77%.From this study, it is clear that trypanosomosis is still a major obstacle to livestock production in Zaria and its Environs andthe incidence is majorly in adult cattle. In addition, nested PCR reaction showed more sensitivity than microscopy. Hence,there is need for proper diagnosis and treatment of animals to promote more beef and milk production for humanconsumption. Further study is necessary to determine the pathogenicity of T. theileri in other domestic animals. Keywords: Trypanosomosis, White Fulani, Sokoto Gudali, breed, infection.
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