Dendrobium liquor eliminates free radicals and suppresses cellular proteins expression disorder to protect cells from oxidant damage
2020; Wiley; Volume: 44; Issue: 12 Linguagem: Inglês
10.1111/jfbc.13509
ISSN1745-4514
AutoresZhang Xue-qin, Ruoxi Zhao, Shigang Zheng, Ze Chun, Yadong Hu,
Tópico(s)Sirtuins and Resveratrol in Medicine
ResumoJournal of Food BiochemistryVolume 44, Issue 12 e13509 FULL ARTICLE Dendrobium liquor eliminates free radicals and suppresses cellular proteins expression disorder to protect cells from oxidant damage Xueqin Zhang, Xueqin Zhang Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China University of Chinese Academy of Sciences, Beijing, China Contribution: Data curation, Investigation, Methodology, Validation, VisualizationSearch for more papers by this authorRuoxi Zhao, Ruoxi Zhao Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Contribution: Data curation, Investigation, Methodology, Validation, VisualizationSearch for more papers by this authorShigang Zheng, Shigang Zheng Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Contribution: ValidationSearch for more papers by this authorZe Chun, Ze Chun Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Contribution: ResourcesSearch for more papers by this authorYadong Hu, Corresponding Author Yadong Hu huyd@cib.ac.cn orcid.org/0000-0003-1704-6980 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Correspondence Yadong Hu, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China. Email: huyd@cib.ac.cn Contribution: Conceptualization, Formal analysis, Project administration, Supervision, Writing - original draft, Writing - review & editingSearch for more papers by this author Xueqin Zhang, Xueqin Zhang Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China University of Chinese Academy of Sciences, Beijing, China Contribution: Data curation, Investigation, Methodology, Validation, VisualizationSearch for more papers by this authorRuoxi Zhao, Ruoxi Zhao Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Contribution: Data curation, Investigation, Methodology, Validation, VisualizationSearch for more papers by this authorShigang Zheng, Shigang Zheng Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Contribution: ValidationSearch for more papers by this authorZe Chun, Ze Chun Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Contribution: ResourcesSearch for more papers by this authorYadong Hu, Corresponding Author Yadong Hu huyd@cib.ac.cn orcid.org/0000-0003-1704-6980 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China Correspondence Yadong Hu, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China. Email: huyd@cib.ac.cn Contribution: Conceptualization, Formal analysis, Project administration, Supervision, Writing - original draft, Writing - review & editingSearch for more papers by this author First published: 06 October 2020 https://doi.org/10.1111/jfbc.13509Citations: 1 Xueqin Zhang and Ruoxi Zhao contributed equally to this work. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Dendrobium liquor obtained by soaking Dendrobium in Chinese liquor is considered as a health drink in China. Here, we found the pretreatment of extract of Dendrobium nobile Lindl. liquor (DNLE) attenuated the oxidative damage to cells caused by H2O2, while the abilities of DNLE of eliminating extracellular free radicals and promoting the activities of intracellular antioxidant enzymes were observed. Quantitative proteomics identified 375 differentially expressed proteins caused by H2O2 treatment in 293T cells. However, only 12 differentially expressed proteins were found in DNLE-pretreated cells which under the same oxidative damage. This suggested that the pretreatment of DNLE could suppress the disorder of protein expressions caused by oxidative stress which could induce cell death. Besides, DNLE was helpful for avoiding the unfolded protein response (UPR) and cell cycle disorder caused by oxidative stress. Taken together, these results demonstrated that Dendrobium liquor could be a healthy herbal drink with antioxidant function. Practical applications Dendrobium is used as an edible herb and a tonic food in traditional Chinese medicine. Dendrobium liquor obtained by soaking Dendrobium with Chinese liquor is also regarded as a nourishing health drink. However, there is rare research data on biological activity of Dendrobium liquor. Our current results demonstrated that the extract of Dendrobium nobile Lindl. liquor (DNLE) possessed the ability of eliminating free radicals in/out the human cells. More importantly, DNLE could help cells to resist the interference on cell life activities caused by oxidative stress. Since many evidences suggested that oxidative stress is linked to human disease and aging, and chemical antioxidant has some side effects on health, Dendrobium liquor can serve as a natural health drink with antioxidant function. Furthermore, the active ingredients in DNLE also possess the potential to be developed as natural antioxidant additive in food and cosmetics. CONFLICT OF INTEREST The authors declare no conflict of interest. Citing Literature Supporting Information Filename Description jfbc13509-sup-0001-TblS1-S3-FigS1-S2.docxWord document, 301.5 KB Table S1-S3-Fig S1-FigS2 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume44, Issue12December 2020e13509 RelatedInformation
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