High-efficiency production of Tremella aurantialba polysaccharide through basidiospore fermentation
2020; Elsevier BV; Volume: 318; Linguagem: Inglês
10.1016/j.biortech.2020.124268
ISSN1873-2976
AutoresTao Sun, Rui Wang, Dafeng Sun, Sha Li, Hong Xu, Yibin Qiu, Peng Lei, Liang Sun, Xiaoqi Xu, Yifan Zhu,
Tópico(s)Microbial Metabolites in Food Biotechnology
ResumoThis study aims to develop a spore fermentation method instead of fruiting body extraction for the production of Tremella aurantialba polysaccharide (TAPS). The purified spore strain Tremella aurantialba NX-20 was isolated for TAPS fermentation. The fermented TAPS and the extracted TAPS had the same monosaccharide composition but higher molecular weight. The conditions for TAPS fermentation by NX-20 were optimized, which were 25 °C, pH 7, 10% initial inoculum and 5 days. To further reduce the processing steps and costs of the medium, tofu wastewater (TW) was used to replace defatted soybean meal (DSM). In a 7.5 L fermentation tank, 40 g/L glucose with 10-fold diluted TW was used as the medium. For TAPS fermentation, a maximum yield of 15.02 ± 0.40 g/L was achieved. In conclusion, this study provides a feasible strategy for the efficient preparation of TAPS through spore fermentation.
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