UHPLC-MS/MS analysis of cannabidiol metabolites in serum and urine samples. Application to an individual treated with medical cannabis
2020; Elsevier BV; Volume: 223; Linguagem: Inglês
10.1016/j.talanta.2020.121772
ISSN1873-3573
AutoresSimona Pichini, Sara Malaca, Massimo Gottardi, Ana Pilar Pérez-Acevedo, Esther Papaseit, Clara Pérez‐Mañá, Magı́ Farré, Roberta Pacifici, Adriano Tagliabracci, Giulio Mannocchi, Francesco Paolo Busardò,
Tópico(s)Alcohol Consumption and Health Effects
ResumoNo analytical assay is currently available for the simultaneous determination of CBD major metabolites in serum or urine samples of individuals treated with medical cannabis or CBD-based pharmaceuticals. We developed and validated a method using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) for quantifying cannabidiol (CBD) and its metabolites, cannabidiol-7-oic acid (7-COOH-CBD), 7- hydroxycannabidiol (7-OH-CBD), 6-alpha-hydroxycannabidiol (6-α–OH–CBD) and 6-beta-hydroxycannabidiol (6-β–OH–CBD) in serum and urine samples of an individual treated with medical cannabis. The ionization was performed by electrospray in negative mode to reach the sensitivity required to detect trace amounts, with limits of quantification ranging from 0.05 to 0.1 ng/mL. The method is accurate (average inter/intra-day error, <15%), precise (inter/intra-day imprecision, <15%) and fast (8 min run time) and it is an essential tool to investigate CBD pharmacokinetics and pharmacodynamics in individuals treated with medical cannabis or with CBD-based medical preparations.
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