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Halomonas lysinitropha sp. nov., a novel halophilic bacterium isolated from a hypersaline wetland

2020; Microbiology Society; Volume: 70; Issue: 12 Linguagem: Inglês

10.1099/ijsem.0.004504

1466-5034

Mohaddaseh Ramezani, Mohammad Pourmohyadini, Mahdi Moshtaghi Nikou, Somaye Makzum, Peter Schümann, Dominique Clermont, Alexis Criscuolo, Mohammad Ali Amoozegar, Peter Kämpfer, Cathrin Spröer,

Bacteriophages and microbial interactions

We carried out a polyphasic taxonomic study on a new halophilic strain designated 3(2) T , isolated from Meighan wetland, Iran. Cells of the novel strain were Gram-stain-negative, non-hemolytic, catalase- and oxidase-positive, rod-shaped, non-endospore-forming and motile. Cell growth occurred at 3–15 % NaCl (w/v; optimum, 5 %), pH 7.0–9.0 (optimum, pH 7.5–8.0) and 15–35 °C (optimum, 30 °C). 16S rRNA gene sequence comparisons confirmed the affiliation of strain 3(2) T to the class Gammaproteobacteria and the genus Halomonas with highest similarity to Halomonas daqiaonensis YCSA28 T (98.4 %) and Halomonas ventosae Al12 T (97.9 %). Experimental and in silico DNA–DNA hybridization values were 42.7 and 35.1% with H. daqiaonensis IBRC-M 10931 T and 48 and 35.2% with H. ventosae IBRC-M 10566 T , respectively, and indicated that they are different members of the same genus. The genome of the type strain was characterized by a size of 3.83 Mbp with 63 scaffolds and a G+C content of 64.8 mol%. Moreover, the average nucleotide identity values against H. ventosae Al12 T and H. daqiaonensis YCSA28 T were 88.8 and 88.5 %, respectively. The predominant respiratory quinone was Q-9 (92 %) with Q-8 (8 %) as a minor component. Major fatty acids were C 16 : 0 cyclo, C 19 : 0 ω8 c , C 16 : 1 ω7 c and/or iso-C 15:0 2-OH, C 12 : 0 3-OH and C 18 : 1 ω7 c . The polar lipid profile of the strain contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphoaminoglycolipid and four unidentified phospholipids. According to our results, strain 3(2) T could be classified as a novel species in the genus Halomonas for which the name Halomonas lysinitropha sp. nov. is proposed. The type strain is 3(2) T (=IBRC M 10929 T =LMG 29450 T =CIP 111708 T ).