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Tempol improves redox status in mdx dystrophic diaphragm muscle

2020; Wiley; Volume: 101; Issue: 6 Linguagem: Inglês

10.1111/iep.12376

1365-2613

Túlio de Almeida Hermes, Daniela Sayuri Mizobuti, Guilherme Luiz da Rocha, Heloina Nathalliê Mariano da Silva, Caroline Covatti, Elaine Cristina Leite Pereira, Renato Ferretti, Elaine Minatel,

Sirtuins and Resveratrol in Medicine

Abstract Oxidative stress is a critical element in relationship to the pathophysiology of Duchenne muscular dystrophy (DMD). In the mice the diaphragm (DIA) is most resembles the dystrophic human pathology. In this study we have evaluated the consequences of a synthetic antioxidant (tempol) on oxidative stress parameters in the DIA muscle of mdx mice. The mdx mice were separated into two groups: mdx, the control group receiving intraperitoneal (i.p.) injections of saline solution (100 µL), and mdxT, the treated group receiving i.p. injections of tempol (100 mg/kg). The tempol‐treated group showed reduced oxidative stress markers, decreasing the dihydroethidium reaction (DHE) area; autofluorescent lipofuscin granules; and 4‐hydroxynonenal (4‐HNE)‐protein adduct levels. DIA muscle of mdx mice. At the same time, the manganese‐superoxide dismutase 2 (SOD2) levels were increased in the tempol‐treated group. In addition, the tempol‐treated group showed reduced levels of glutathione‐disulphide reductase (GSR), glutathione peroxidase 1 (GPx1) and catalase (CAT) in immunoblots. The tempol‐treated group has also shown lower relative gene expression of SOD1, CAT and GPx than the non‐treated group. Our data demonstrated that tempol treatment reduced oxidant parameters and increased anti‐oxidant SOD2 levels in the DIA muscle of mdx mice, which may contribute to the normalization of the redox homeostasis of dystrophic muscles.