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Multimodal mapping of the tumor and peripheral blood immune landscape in human pancreatic cancer

2020; Nature Portfolio; Volume: 1; Issue: 11 Linguagem: Inglês

10.1038/s43018-020-00121-4

2662-1347

Nina G. Steele, Eileen S. Carpenter, Samantha B. Kemp, Veerin R. Sirihorachai, Stephanie The, Lawrence Delrosario, Jenny Lazarus, El-ad David Amir, Valerie Gunchick, Carlos E. Espinoza, Samantha L. Bell, Lindsey Harris, Fatima Lima, Valerie Irizarry-Negron, Daniel Paglia, Justin Macchia, Angel Ka Yan Chu, Heather Schofield, Erik-Jan Wamsteker, Richard S. Kwon, Allison R. Schulman, Anoop Prabhu, Ryan Law, Arjun R. Sondhi, Jessica Yu, Arpan Patel, Katelyn L. Donahue, Hari Nathan, Clifford Cho, Michelle A. Anderson, Vaibhav Sahai, Costas A. Lyssiotis, Weiping Zou, Benjamin L. Allen, Arvind Rao, Howard C. Crawford, Filip Bednar, Timothy L. Frankel, Marina Pasca di Magliano,

Cancer Immunotherapy and Biomarkers

Pancreatic ductal adenocarcinoma (PDA) is characterized by an immune-suppressive tumor microenvironment that renders it largely refractory to immunotherapy. We implemented a multimodal analysis approach to elucidate the immune landscape in PDA. Using a combination of CyTOF, single-cell RNA sequencing, and multiplex immunohistochemistry on patient tumors, matched blood, and non-malignant samples, we uncovered a complex network of immune-suppressive cellular interactions. These experiments revealed heterogeneous expression of immune checkpoint receptors in individual patient's T cells and increased markers of CD8