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Photobiomodulation drives pericyte mobilization towards skin regeneration

2020; Nature Portfolio; Volume: 10; Issue: 1 Linguagem: Inglês

10.1038/s41598-020-76243-7

2045-2322

Isabella Bittencourt do Valle, Pedro H. D. M. Prazeres, Ricardo Alves Mesquita, Tarcı́lia Aparecida Silva, Hortência Maciel de Castro Oliveira, Pollyana Ribeiro Castro, Iuri Dornelas Prates Freitas, Sicília Rezende Oliveira, Nelson Pinto Gomes, Rafaela Férrer de Oliveira, Larissa Fassarela Marquiore, Soraia Macari, Flávio Almeida do Amaral, Humberto Jácome‐Santos, Lucíola S. Barcelos, Gustavo Batista Menezes, Márcia Martins Marques, Alexander Birbrair, Ivana Márcia Alves Diniz,

Wound Healing and Treatments

Abstract Photobiomodulation is being widely applied for improving dermal or mucosal wound healing. However, the underlying cellular and molecular processes that directly contribute to its effects remain poorly understood. Pericytes are relevant cells involved in the wound microenvironment and could be one of the main targets of photobiomodulation due to their plasticity and perivascular localization. Herein, we investigate tissue repair under the photobiomodulation stimulus using a pericyte labeled (or reporter) transgenic mice. Using a model of two contralateral back wounds, one the control and the other photoactivated daily (660 nm, 20 mW, 0.71 W/cm 2 , 5 J/cm 2 , 7 s, 0.14 J), we showed an overall influx of immune and undifferentiated cells and higher mobilization of a potent pericyte subpopulation (Type-2 pericytes) in the photoactivated wounds in comparison to the controls. Doppler analysis showed a significant increase in the blood flow in the photoactivated wounds, while marked vascular supply was observed histologically. Histochemical analysis has indicated more advanced stages of tissue repair after photoactivation. These data suggest that photobiomodulation significantly accelerates tissue repair through its vascular effects with direct recruitment of pericytes to the injury site.