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Resumption and progression of meiosis and circulating levels of steroids and prostaglandin F 2α of Piaractus mesopotamicus induced by hypophysation with prostaglandin F 2α
2020; Wiley; Volume: 52; Issue: 3 Linguagem: Inglês
10.1111/are.14957
ISSN1365-2109
AutoresRafael Tomoda Sato, Rafael Yutaka Kuradomi, Marina Cabral Calil, Laíza Maria de Jesus Silva, Mariana Roza de Abreu, Daniel Guimarães Figueiredo‐Ariki, Guilherme Antônio de Freitas, Sérgio Ricardo Batlouni,
Tópico(s)Aquaculture Nutrition and Growth
ResumoAquaculture ResearchVolume 52, Issue 3 p. 1026-1037 ORIGINAL ARTICLE Resumption and progression of meiosis and circulating levels of steroids and prostaglandin F2α of Piaractus mesopotamicus induced by hypophysation with prostaglandin F2α Rafael Tomoda Sato, Rafael Tomoda Sato orcid.org/0000-0003-2422-6875 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorRafael Yutaka Kuradomi, Rafael Yutaka Kuradomi orcid.org/0000-0002-8579-2750 Institute of Exact Sciences and Technology-ICET, Federal University of Amazonas-UFAM, Itacoatiara, BrazilSearch for more papers by this authorMarina Cabral Calil, Marina Cabral Calil Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorLaiza Maria de Jesus Silva, Laiza Maria de Jesus Silva orcid.org/0000-0002-1753-2543 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorMariana Roza de Abreu, Mariana Roza de Abreu orcid.org/0000-0001-8675-1101 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorDaniel Guimarães Figueiredo-Ariki, Daniel Guimarães Figueiredo-Ariki orcid.org/0000-0002-8220-7042 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorGuilherme Antônio de Freitas, Guilherme Antônio de Freitas orcid.org/0000-0002-3690-7769 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorSergio Ricardo Batlouni, Corresponding Author Sergio Ricardo Batlouni sergio.ricardo@unesp.br orcid.org/0000-0003-3579-2530 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, Brazil Correspondence Sergio Ricardo Batlouni, Aquaculture Center of São Paulo State University–CAUNESP, São Paulo State University–UNESP, Via de Acesso Prof. Paulo Donato Castellane, S/N, Jaboticabal, SP 14884-900, Brazil. Email: sergio.ricardo@unesp.brSearch for more papers by this author Rafael Tomoda Sato, Rafael Tomoda Sato orcid.org/0000-0003-2422-6875 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorRafael Yutaka Kuradomi, Rafael Yutaka Kuradomi orcid.org/0000-0002-8579-2750 Institute of Exact Sciences and Technology-ICET, Federal University of Amazonas-UFAM, Itacoatiara, BrazilSearch for more papers by this authorMarina Cabral Calil, Marina Cabral Calil Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorLaiza Maria de Jesus Silva, Laiza Maria de Jesus Silva orcid.org/0000-0002-1753-2543 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorMariana Roza de Abreu, Mariana Roza de Abreu orcid.org/0000-0001-8675-1101 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorDaniel Guimarães Figueiredo-Ariki, Daniel Guimarães Figueiredo-Ariki orcid.org/0000-0002-8220-7042 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorGuilherme Antônio de Freitas, Guilherme Antônio de Freitas orcid.org/0000-0002-3690-7769 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, BrazilSearch for more papers by this authorSergio Ricardo Batlouni, Corresponding Author Sergio Ricardo Batlouni sergio.ricardo@unesp.br orcid.org/0000-0003-3579-2530 Aquaculture Center of São Paulo State University, CAUNESP, São Paulo State University–UNESP, Sao Paulo, Brazil Correspondence Sergio Ricardo Batlouni, Aquaculture Center of São Paulo State University–CAUNESP, São Paulo State University–UNESP, Via de Acesso Prof. Paulo Donato Castellane, S/N, Jaboticabal, SP 14884-900, Brazil. Email: sergio.ricardo@unesp.brSearch for more papers by this author First published: 02 November 2020 https://doi.org/10.1111/are.14957Citations: 2Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract We have previously shown an increase on the ovulation rate of pacu, Piaractus mesopotamicus, using hypophysation with prostaglandin F2α (PGF2α) (HPG protocol) rather than hypophysation (H); only, however, a persistent lack of ovulation in some females could not be further explained by PGF2α and steroid plasma levels at time of ovulation. Considering this, here we compared the meiotic process and steroids and PGF2α levels during spawning induction using both protocols. To that, H and HPG were treated with conventional hypophysation (0.6 mg/kg priming and 5.4 mg/kg resolving dose), and the latter received synthetic PGF2α (2 mL/fish) at resolving dose. A negative control (saline solution), a SPG group (receiving only PGF2α) and a PPG group (receiving priming and PGF2α) were included. An initial PGF2α peak between one to four hours post-resolving dose (1-4 hr) occurred in groups receiving PGF2α (HPG, SPG and PPG). Only H and HPG showed a 17α-20β-dihydroxy-4-pregnen-3-one (DHP) peak 4 hr, concomitant with meiosis resumption, and a final PGF2α peak 12 hours. The HPG protocol resulted in higher PGF2α plasma levels for longer periods (1-4 and 12 hr) than H (single peak 12 hr), occasioning in the former higher and concomitant levels of both PGF2α and DHP at 4 h. Considering that DHP is known to induce expression of prostaglandin receptors in fish, the concomitant plasma peaks of DHP and PGF2α may also explain the ovulation rate gain previously published, using HPG protocol, which can be further improved adjusting the priming dose and defining the exact interval of PGF2α ovulatory action in this species. CONFLICT OF INTEREST Authors do not have any conflict of interest to declare. Open Research DATA AVAILABILITY STATEMENT The data are in the master's thesis model and are available to the public at http://hdl.handle.net/11449/153272. Citing Literature Supporting Information Filename Description are14957-sup-0001-FigS1.pngPNG image, 1.5 MB Figure S1 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume52, Issue3March 2021Pages 1026-1037 RelatedInformation
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