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Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer

2020; De Gruyter; Volume: 40; Issue: 3 Linguagem: Croata

10.5937/jomb0-27945

1452-8258

Ozmen Unalli, Yeşim Özarda,

Bacterial Identification and Susceptibility Testing

The complete blood count (CBC) with differential leukocyte count (DLC) is one of the most common tests requested by physicians. The results of this test are affected by storage temperature and time of incubation. This study was designed to evaluate the stability of hematologic parameters in blood specimens stored for 48 h at three temperatures.K2-EDTA - blood was collected from 22 healthy adults. The CBC was performed using a hematology analyser immediately; 0 time point and at 4, 8, 12, 16, 20, 24, and 48 h after storage at 4 °C, 10 °C or 23 °C. Changes in values of CBC parameters from the 0 time point were determined and reported as % of the initial value.Red blood cells, platelet, hemoglobin, and mean corpuscular hemoglobin were found stable during 48 h storage at 4 °C, 10 °C or 23 °C. Hematocrite and mean corpuscular volume increased, while white blood cells decreased at 48 h when stored at 23 °C. Lymphocytes, neutrophils, eosinophils, and basophils showed significant differences after 12 h of storage at 23 °C.Red blood cells, platelet, hemoglobin, and mean corpuscular hemoglobin are the only suitable parameters without refrigeration during 24 h storage. When CBC and DLC are performed, 4 °C can be recommended as the most suitable storage temperature for 12 h storage.Kompletna krvna slika (CBC) sa diferencijalnim brojem leukocita (DLC) jedan je od najčešćih testova koji zahtevaju lekari. Na rezultate ovog testa utiču temperatura skladištenja i vreme inkubacije. Ova studija je osmišljena da proceni stabilnost hematoloških parametara u uzorcima krvi čuvanim 48 sati na tri temperature.K2-EDTA - krv je uzeta od 22 zdrave odrasle osobe. Odmah je određena kompletna krvna slika pomoću hematološkog analizatora; nulta vremenska tačka i na 4, 8, 12, 16, 20, 24 i 48 sati nakon skladištenja na 4 °C, 10 °C ili 23 °C. Promene u vrednostima CBC parametara u odnosu na nultu vremensku tačku su utvrđene i navedene kao % od početne vrednosti.Eritrociti, trombociti, hemoglobin i srednji korpuskularni hemoglobin bili su stabilni tokom 48 sati skladištenja na 4 °C, 10 °C ili 23 °C. Hematokrit i srednja korpuskularna zapremina su se povećavali, dok su se leukociti smanjili tokom 48 sati kada su bili uskladištene na 23 °C. Limfociti, neutrofili, eozinofili i bazofili pokazali su značajne razlike nakon 12 sati skladištenja na 23 °C.Eritrociti, trombociti, hemoglobin i srednji korpuskularni hemoglobin su jedini pogodni parametri koji se mogu uzeti u obzir bez hlađenja tokom 24-časovnog skladištenja. Kada se rade CBC i DLC, tempe ratura od 4 °C se može preporučiti kao najpogodnija za 12 sati skladištenja.