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Implications of Topoisomerase (TOP1 and TOP2α) Expression in Patients With Breast Cancer

2020; Stanford University Highwire Press; Volume: 34; Issue: 6 Linguagem: Inglês

10.21873/invivo.12188

1791-7549

Misato Ogino, Takaaki Fujii, Yuko Nakazawa, Toru Higuchi, Yukio Koibuchi, Tetsunari Oyama, Jun Horiguchi, Ken Shirabe,

Advanced biosensing and bioanalysis techniques

We evaluated the usefulness of topoisomerases (TOPs) expression as prognostic predictors in breast cancer.We retrospectively investigated sixty cases with primary breast cancer. We evaluated the tumor and non-tumor mRNA levels of TOP1 and TOP2α using quantitative reverse-transcription polymerase chain reaction. TOP1/TOP2α positivity was defined as the ratio of the mRNA expression of cancer/normal tissue of >1 for both TOP1 and TOP2α.TOP1 and TOP2α were markedly overexpressed in breast cancer tissues compared to normal breast tissues. Of the 60 cases, 46 (76.7%) were positive for TOP1/TOP2α. The relapse-free survival was relatively shorter for patients with positive TOP1/TOP2α. There was no recurrent disease among the 14 patients who were negative for TOP1/TOP2α, whereas four of the 46 TOP1/TOP2α-positive patients had disease recurrence.Negative TOP1 or TOP2α expression may be useful for predicting better prognoses in breast cancer patients.