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Genomic sequencing of two isolates of Ralstonia solanacearum causing Sergipe facies and comparative analysis with Bugtok disease isolates

2020; Brazilian Society of Genetics; Volume: 43; Issue: 4 Linguagem: Inglês

10.1590/1678-4685-gmb-2020-0155

1678-4685

Jéssica Rodrigues da Silva, Ana Karolina Leite Pais, Greecy Mirian Rodrigues Albuquerque, Adriano Márcio Freire Silva, Wilson José Silva, Valdir de Queiroz Balbino, M. E. N. Fonseca, Marco Aurélio Siqueira da Gama, Elineide Barbosa de Souza, Rosa de Lima Ramos Mariano,

Genomics and Phylogenetic Studies

Ralstonia solanacearum is the causal agent of Moko disease in bananas, which in the state of Sergipe in northeastern Brazil causes "Sergipe facies" .This disease induces atypical symptoms similar to those of Bugtok disease in the Philippines.This study was conducted to sequence, assemble, and annotate the genomes of the Sergipe faciescausing isolates SFC and IBSBF2570 (sequevar IIA-53) and compare their genomes with two representative isolates causing Bugtok disease.The genomes were sequenced and assembled, resulting in lengths of 5.58 Mb (SFC) and 5.46 Mb (IBSBF2570) in 185 and 174 contigs, respectively.The isolates of Sergipe facies and Bugtok disease showed similarities in their gene contents.We identified 5,668 information clusters, 3,752 of which were shared by all genomes (core genes).Moreover, 3,585 single-copy genes were identified.Isolates causing Bugtok disease exclusively shared 266 more information clusters than the isolates causing Sergipe facies.These results suggest that Sergipe facies and Bugtok disease isolates show high genomic similarity.However, the similarity is even greater between the Bugtok disease isolates.This may be because of their longer period of interaction compared to Sergipe facies isolates.