Comments on “Hyaluronidase: an overview of its properties, applications, and side effects”
2020; Thieme Medical Publishers (Germany); Volume: 47; Issue: 06 Linguagem: Inglês
10.5999/aps.2020.01571
ISSN2234-6171
Autores Tópico(s)Proteoglycans and glycosaminoglycans research
ResumoAn overview of its properties, applications, and side effects" [1].We would like to express our respect for the author for this well-organized review article.However, there are a few points that we would like to discuss.In the section "Action of hyaluronidase," the author stated that "Hyaluronidase is an endoglycosidase that breaks down hyaluronic acid into monosaccharides" [1].As described in the article, hyaluronidase breaks β-1,4 glycosidic bonds or β-1,3 glycosidic bonds in a hyaluronic acid chain.However, a hyaluronic acid chain is composed of multiple disaccharide chains and, when hyaluronidase cleaves the β-1,4 or β-1,3 glycosidic bonds of hyaluronic acid, the chain breaks down to form disaccharides, not monosaccharides.The author stated that "microbial hyaluronidase obtained from Streptococcus agalactiae bacteria was also used to reduce side effects" [1].As far as we know, many hyaluronidase products are still made from animal testicular preparations.For example, Vitrase (ISTA Pharmaceuticals, Inc., Irvine, CA, USA) is formulated as a solution containing ovine hyaluronidase, Hylenex (Halozyme Therapeutics, Inc., San Diego, CA, USA) is human recombinant hyaluronidase formed by genetically engineered Chinese hamster ovary cells containing a DNA plasmid encoding the enzyme, Liporase (Daehan New Pharm Co., Seoul, Korea) is made from a bovine testicular preparation, and Hirax (BMI Korea, Uiwamg, Korea) is also made from a bovine testicular preparation.The author stated that "The reasons for the short half-life of hyaluronidase in human plasma are the presence of numerous hyaluronidase inhibitors in the plasma" [1].However, this has not been proven by any scientific study.A previous study observed that hyaluronidase
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