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Production of Lentiviral Vectors

2002; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-3-642-56114-6_5

2196-9965

Romain Zufferey,

RNA Interference and Gene Delivery

The interest in lentiviral vectors as tools for gene therapy has increased dramatically over the last few years. The number of published studies on lentiviral vectors reflects this growing interest. Whereas only four papers were published in 1996, more than 40 publications appeared in 2000. The vast majority of these papers deal with the transduction of different cell types from different species either in vitro or in vivo. A few of them propose new vector designs with enhanced efficacy or higher safety. Only very few address the practical problems of vector production and purification. However, as the proofs for efficient transgene delivery and sustained expression in a variety of cell types accumulate, methods for the large-scale production of clinical grade vector stocks become urgently needed. Recently, significant progress has been made toward the development of efficient production systems. The efforts should focus now on the processing downstream from the production, mainly on the purification of the vector particles. This chapter presents the current state of the art in this field. Emphasis will be on the production of HIV-1-based vectors because this is the most frequently used and the best understood system.