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Proteome responses of Rhizobium tropici CIAT 899 upon apigenin and salt stress induction

2020; Elsevier BV; Volume: 159; Linguagem: Inglês

10.1016/j.apsoil.2020.103815

1873-0272

Mariana Rocha Maximiano, Esaú Megías, Ivonaldo Reis Santos, Lucas Souza dos Santos, Francisco Javier Ollero, Manuel Megı́as, Octávio Luiz Franco, Ângela Mehta,

Plant-Microbe Interactions and Immunity

Rhizobium tropici establishes symbiosis with several leguminous species, inducing nitrogen fixing nodules. Communication between rhizobia and legumes starts when the flavonoids secreted by plant roots interact with the R. tropici NodD protein to recognize plant signaling molecules present in the rhizosphere and begin the nodulation process. Some studies in R. tropici CIAT 899 have demonstrated that the induction of nod genes by saline stress influences the formation of nodules in plant roots. The aim of this study was to analyze the proteome profile of R. tropici CIAT 899 to investigate the molecular response of the bacterium under apigenin (flavonoid) and salt stress induction. The bacterium was grown in TY medium, considered as the control condition (CC), and in the presence of apigenin (AC) and NaCl (SSC). Total proteins were extracted and analyzed by two-dimensional electrophoresis (2-DE) and the differential spots were identified by MALDI-TOF MS/MS. A total of 105 differentially abundant protein spots were observed, including 2 present only in CC, 2 exclusive to AC and 6 to SSC. A total of 73% of these protein spots were identified by mass spectrometry. Proteins involved in transport and cellular processes were mostly increased in AC:CC and AC:SSC comparison, while in SSC:CC, these proteins were mostly decreased. Under salt stress, the bacterium reduced the uptake of molecules and energy consumption, reducing the abundance of ABC transporter proteins. These proteins are crucial for bacterial adaptation in environmental conditions and can be important for the nodulation process.