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Research Article Analysis of microRNAs -15b, -16, -21, -221 and -222 as molecular markers in the blood of rats submitted to focal cerebral ischemia associated with alcoholism

2020; Research Foundation of Ribeirão Preto; Volume: 19; Issue: 3 Linguagem: Inglês

10.4238/gmr18637

1676-5680

Paulo Cézar Novais, Fermino Sanches Lizarte Neto, Múcio Luiz de Assis Cirino, Lucas Barbosa Porsani, J.P. da Silva, Isabella Stracieri Gula, de T. Durand, Camila Albuquerque Melo de Carvalho, Bruno César Schimming, de F.G.S. Tazima, Carlos Gilberto Carlotti, Benedito Oscar Colli, Daniela Pretti da Cunha Tirapelli, Luís Fernando Tirapelli,

Neurological Disease Mechanisms and Treatments

IR), Alcoholic (A); Alcoholic and ischemic (I + A); Ischemiareperfusion and Alcoholic (IR+A).The blood samples were collected for gene expression analysis of some serum miRNAs by PCR in real time.The serum expression of miRNA-16 was higher in the IR group compared to C and S groups (P < 0.05) but no association with chronic alcoholism was found.The serum expressions of miRNA-21, -221 and -222 were low in all groups and were not correlated with ischemic injury and/or chronic alcoholism.The serum expressions of miRNA-15b, -21, -221 and -222 were similar among the experimental groups, with no correlation with ischemia, with or without reperfusion, and/or alcoholism.The overexpression of miRNA-16 in the blood of I and IR groups demonstrated a correlation with the ischemic process, mainly after reperfusion for 48 hours, associated or not with alcoholism.