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Anesthetic potential of the essential oils of Aloysia triphylla, Lippia sidoides and Mentha piperita for Colossoma macropomum

2020; Elsevier BV; Volume: 534; Linguagem: Inglês

10.1016/j.aquaculture.2020.736275

1873-5622

Franmir Rodrigues Brandão, Caio Francisco Santana Farias, Damy Caroline de Melo Souza, Maria Inês Braga de Oliveira, L. Matos, Cláudia Majolo, M. R. de Oliveira, Francisco Célio Maia Chaves, Fernanda Loureiro de Almeida, Edsandra Campos Chagas,

Turtle Biology and Conservation

The aim of this study was to evaluate the anesthetic potential of the essential oils of Aloysia triphylla (EOAT), Lippia sidoides (EOLS) and Mentha piperita (EOMP) in relation to juvenile tambaqui (Colossoma macropomum), based the swimming behavior of these fish and possible histopathological damage to the gills. To induce anesthesia, juvenile tambaqui (130.55 ± 22.4 g; 16.8 ± 0.95 cm) were individually exposed to different concentrations of the essential oils (EOAT: 5, 10, 20, 40, 60, 80 and 150 mg L−1; EOLS: 2, 5, 10, 20, 30, 40 and 50 mg L−1; EOMP: 5, 10, 20, 40, 60, 70 and 90 mg L−1), for exposure periods of between four minutes and six hours. The three essential oils evaluated presented anesthetic properties. The concentration intervals over which the fish reached all the stages of anesthesia (1, 2, 3a, 3b and 4) were between 40 and 150 mg L−1 for EOAT; 20 and 50 mg L−1 for EOLS; and 40 and 90 mg L−1 for EOMP. The times taken to reach the anesthesia stages were inversely proportional to the increases in the concentration of the essential oils. The histopathological damage to the gills of the tambaqui that resulted from using essential oil concentrations that were defined as anesthetic (150 mg L−1 EOAT, 30 mg L−1 EOLS and 90 mg L−1 EOMP) were also evaluated. The main damage recorded was hypertrophy and hyperplasia of the lamellar epithelium, lamellar fusion and proliferation of chloride cells. According to the histopathological alteration index (HAI), there were no irreversible damage (such as fibrosis and necrosis) to the gills of the tambaqui. However, while EOLS and EOMP caused mild damages in the gills, the EOAT generated more severe damages. Overall, for the tambaqui, the essential oils of A. triphylla, L. sidoides and M. piperita are efficient anesthetic in the doses of 150, 30 and 90 mg L−1, respectively.