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BIBTEX RIS

Mini-review: from in vitro to ex vivo studies: an overview of alternative methods for the study of medical biofilms

2020; Taylor & Francis; Linguagem: Inglês

10.1080/08927014.2020.1859499

1029-2454

Débora de Souza Collares Maia Castelo‐Branco, Bruno Rocha Amando, Crister José Ocadaque, Lara de Aguiar, Débora Damásio de Queiroz Paiva, Expedito Maia Diógenes, Gláucia Morgana de Melo Guedes, Cecília Leite Costa, Anísio Silvestre Pinheiro Santos-Filho, Ana Raquel Colares de Andrade, Rossana de Aguiar Cordeiro, Marcos Fábio Gadelha Rocha, José Júlio Costa Sidrim,

Antimicrobial Peptides and Activities

Microbial biofilms are a natural adaptation of microorganisms, typically composed of multiple microbial species, exhibiting complex community organization and cooperation. Biofilm dynamics and their complex architecture are challenging for basic analyses, including the number of viable cells, biomass accumulation, biofilm morphology, among others. The methods used to study biofilms range from in vitro techniques to complex in vivo models. However, animal welfare has become a major concern, not only in society, but also in the academic and scientific field. Thus, the pursuit for alternatives to in vivo biofilm analyses presenting characteristics that mimic in vivo conditions has become essential. In this context, the present review proposes to provide an overview of strategies to study biofilms of medical interest, with emphasis on alternatives that approximate experimental conditions to host-associated environments, such as the use of medical devices as substrata for biofilm formation, microcosm and ex vivo models.