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Mutations Found in the Asc1 Gene That Confer Susceptibility to the AAL-Toxin in Ancestral Tomatoes from Peru and Mexico

2020; Multidisciplinary Digital Publishing Institute; Volume: 10; Issue: 1 Linguagem: Inglês

10.3390/plants10010047

2223-7747

Rin Tsuzuki, Rosa María Cabrera Pintado, Jorge Andrés Biondi Thorndike, Dina Lida Gutiérrez Reynoso, Carlos A. Amasifuén Guerra, Juan Carlos Guerrero‐Abad, L. Aragón-Caballero, Medali Heidi Huarhua Zaquinaula, Cledy Ureta Sierra, Olenka Ines Alberca Cruz, Milca Gianira Elespuru Suna, Raúl Blas, Ines Carolina Torres Arias, Joel Flores Ticona, Fatima Cáceres de Baldarrago, Juan Enrique Rodríguez-Pérez, Takuo Hozum, Hiroki Saito, Shunsuke Kotera, Yasunori Akagi, Motoichiro Kodama, Ken Komatsu, Tsutomu Arie,

Plant pathogens and resistance mechanisms

Tomato susceptibility/resistance to stem canker disease caused by Alternaria alternata f. sp. lycopersici and its pathogenic factor AAL-toxin is determined by the presence of the Asc1 gene. Several cultivars of commercial tomato (Solanum lycopersicum var. lycopersicum, SLL) are reported to have a mutation in Asc1, resulting in their susceptibility to AAL-toxin. We evaluated 119 ancestral tomato accessions including S. pimpinellifolium (SP), S. lycopersicum var. cerasiforme (SLC) and S. lycopersicum var. lycopersicum “jitomate criollo” (SLJ) for AAL-toxin susceptibility. Three accessions, SP PER018805, SLC PER018894, and SLJ M5-3, were susceptible to AAL-toxin. SLC PER018894 and SLJ M5-3 had a two-nucleotide deletion (nt 854_855del) in Asc1 identical to that found in SLL cv. Aichi-first. Another mutation (nt 931_932insT) that may confer AAL-toxin susceptibility was identified in SP PER018805. In the phylogenetic tree based on the 18 COSII sequences, a clade (S3) is composed of SP, including the AAL-toxin susceptible PER018805, and SLC. AAL-toxin susceptible SLC PER018894 and SLJ M5-3 were in Clade S2 with SLL cultivars. As SLC is thought to be the ancestor of SLL, and SLJ is an intermediate tomato between SLC and SLL, Asc1s with/without the mutation seem to have been inherited throughout the history of tomato domestication and breeding.