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EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

2020; Elsevier BV; Volume: 104; Linguagem: Inglês

10.1016/j.ijid.2020.12.051

1878-3511

Stefania Paolucci, Irene Cassaniti, Federica Novazzi, L Fiorina, Antonio Piralla, Giuditta Comolli, Raffaele Bruno, Renato Maserati, Roberto Gulminetti, Stefano Novati, Francesco Mojoli, Fausto Baldanti, Raffaele Bruno, Mario U. Mondelli, Enrico Brunetti, Angela Di Matteo, Elena Seminari, Laura Maiocchi, Valentina Zuccaro, Layla Pagnucco, B Mariani, Serena Ludovisi, Raffaella Lissandrin, Antonio Parisi, Paolo Sacchi, SFA Patruno, G Michelone, Roberto Gulminetti, Domenico Zanaboni, Stefano Novati, Renato Maserati, P Orsolini, Marco Vecchia, Marco Sciarra, Erika Asperges, Marta Colaneri, Di Filippo A, Margherita Sambo, Simona Biscarini, Matteo Lupi, Silvia Roda, T.C. Pieri, Ilaria Gallazzi, Michele Sachs, P. Valsecchi, Stefano Perlini, C Alfano, Marco Bonzano, Domenica Federica Briganti, Giuseppe Crescenzi, A.G. Falchi, Roberta Guarnone, Barbara Katia Guglielmana, Enrico Maggi, Ilaria Martino, Pietro Pettenazza, Serena Pioli di Marco, Francesca Maria Quaglia, Anna Sabena, Francesco Salinaro, Francesco Speciale, Ilaria Zunino, M De Lorenzo, Gioel Gabrio Secco, L. Dimitry, Giovanni Cappa, Igor Maisak, Benedetta Chiodi, M. Sciarrini, Bruno Barcella, Flavia Resta, Luca Moroni, Giulia Vezzoni, Lorenzo Scattaglia, Enrico Boscolo, Caterina Zattera, MF Tassi, Vincenzo Capozza, Damiano Vignaroli, Marco Bazzini, Giorgio Antonio Iotti, Francesco Mojoli, Mirko Belliato, Luciano Perotti, Silvia Mongodi, Guido Tavazzi, Gian Luigi Marseglia, Amelia Licari, Ilaria Brambilla, Daniela Barbarini, Antonio Bruno, Patrizia Cambieri, Giulia Campanini, C. Cavanna, Giuditta Comolli, Marta Corbella, R. Daturi, Milena Furione, B Mariani, P. Marone, Renato Maserati, E. Monzillo, Stefania Paolucci, M. Parea, Elena Percivalle, Antonio Piralla, Francesca Rovida, Antonella Sarasini, Maurizio Zavattoni, G. Adzasehoun, Marco Vittorio Rossi Ardizzone, Laura Bellotti, Vincenzo Brunco, Ermanna Cabano, Gianluca Casali, L Capella, D Devitis, Luca Dossena, G Frisco, G. Garbagnoli, F Gardellini, Alessia Girello, A Guerrizio, V. Landini, C. Lucchelli, V. Maliardi, Pasqua Piemontese, S. Pezzaia, María Sol Prémoli, Chiara Rebuffa, Cinzia Zanello, Jessica Bagnarino, Federica Bergami, Antonella Bonetti, G Caneva, Irene Cassaniti, Antonio Corcione, Raffaella Di Martino, Anna Napoli, Alessandro Ferrari, Guglielmo Ferrari, L Fiorina, Anna Gallone, Federica Giardina, Adriana Castello Costa Girardi, A Mercato, Cristina Merla, F Novazzi, G. Ratano, Barbara Rossi, G Saveriaempillai, I.M. Sciabica, Monica Tallarita, Edoardo Vecchio Nepita, Julian Vitali, Antonella Cerino, Stefania Varchetta, Barbara Oliviero, Stefania Mantovani, Dalila Mele, Monica Calvi, Michele Tizzoni, Carlo Nicora, Antonio Triarico, Vincenzo Petronella, Carlo Marena, A Muzzi, Paolo Lago, Sara Cutti, Viola Novelli, Francesco Comandatore, Gherard Batisti Biffignandi, Stefano Gaiarsa, Marco Rettani, Claudio Bandi, Alessandro Ferrari,

Parvovirus B19 Infection Studies

Highlights•EBV reactivation was observed in patients with COVID-19.•NK+and CD8+T cell reduction was related to the severity of COVID-19 infection.•An increase of B cells in patients with severe symptoms was documented.AbstractObjectivesThe immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity.MethodsA total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry.ResultsAmong opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p<0.05). In contrast, B cell count was significantly increased in ICU patients (p=0.0172).ConclusionsA correlation between reduced CD8+ T cells and NK counts, EBV DNA levels and COVID-19 severity was observed. Other opportunistic viral infections were not observed. The relationship between EBV load and COVID-19 severity should be further evaluated in longitudinal studies.