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Hyper-production optimization of fungal oxidative green enzymes using citrus low-cost byproduct

2020; Elsevier BV; Volume: 9; Issue: 1 Linguagem: Inglês

10.1016/j.jece.2020.105013

2213-3437

Débora da Silva Vilar, Clara Dourado Fernandes, Victor Ruan Silva Nascimento, Nádia Hortense Torres, Manuela Souza Leite, Ram Naresh Bharagava, Muhammad Bilal, Giancarlo R. Salazar‐Banda, Katlin I.B. Eguiluz, Luiz Fernando Romanholo Ferreira,

Biochemical and biochemical processes

The use of alternative methods is necessary to improve the production of lignin-modifying enzymes (LMEs) in the biocatalysis scenario. This study demonstrates a new and robust optimization for Lac and MnP enzymes production from Pleurotus sajor-caju induced by pulp wash citrus byproduct. The optimal values determined through response surface methodology (RSM) and artificial neural network coupled to the genetic algorithm (ANN-GA) were agitation rate (180 rpm) and pH (5.5), with a temperature of 28 ºC, after 8 days of incubation. The maximum production of Lac and MnP obtained in these conditions using RSM was 307,379.91 IU/L (R2 = 0.9566) and 11,890.20 IU/L (R2 = 0.9932), respectively. However, ANN-GA predicted a maximum production of 204,486.96 IU/L and 36,081.02 UI/L, with R2 of 0.9903. Thus, under optimized conditions, the agroindustrial pulp wash residue emerges as a promising substrate in the production of LMEs, and therefore, its biotechnological viability enables efficient and sustainable production of bioproducts.